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Altered glucose catabolism in the presynaptic and perisynaptic compartments of SOD1G93Amouse spinal cord and motor cortex indicates that mitochondria are the site of bioenergetic imbalance in ALS
- Source :
- Journal of Neurochemistry. 151:336-350
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Amyotrophic lateral sclerosis is an adult-onset neurodegenerative disease that develops because of motor neuron death. Several mechanisms occur supporting neurodegeneration, including mitochondrial dysfunction. Recently, we demonstrated that the synaptosomes from the spinal cord of SOD1G93A mice, an in vitro model of presynapses, displayed impaired mitochondrial metabolism at early pre-symptomatic stages of the disease, whereas perisynaptic astrocyte particles, or gliosomes, were characterized by mild energy impairment only at symptomatic stages. This work aimed to understand whether mitochondrial impairment is a consequence of upstream metabolic damage. We analyzed the critical pathways involved in glucose catabolism at presynaptic and perisynaptic compartments. Spinal cord and motor cortex synaptosomes from SOD1G93A mice displayed high activity of hexokinase and phosphofructokinase, key glycolysis enzymes, and of citrate synthase and malate dehydrogenase, key Krebs cycle enzymes, but did not display high lactate dehydrogenase activity, the key enzyme in lactate fermentation. This enhancement was evident in the spinal cord from the early stages of the disease and in the motor cortex at only symptomatic stages. Conversely, an increase in glycolysis and lactate fermentation activity, but not Krebs cycle activity, was observed in gliosomes from the spinal cord and motor cortex of SOD1G93A mice although only at the symptomatic stages of the disease. The cited enzymatic activities were enhanced in spinal cord and motor cortex homogenates, paralleling the time-course of the effect observed in synaptosomes and gliosomes. The observed metabolic modifications might be considered an attempt to restore altered energetic balance and indicate that mitochondria represent the ultimate site of bioenergetic impairment.
- Subjects :
- 0301 basic medicine
Gliosomes
Mitochondrion
Biochemistry
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
medicine
Citrate synthase
Glycolysis
biology
Chemistry
Neurodegeneration
Motor Cortex
Motor neuron
medicine.disease
Spinal cord
Cell biology
Citric acid cycle
030104 developmental biology
medicine.anatomical_structure
Spinal Cord
biology.protein
Krebs cycle
Synaptosomes
030217 neurology & neurosurgery
Astrocyte
Subjects
Details
- ISSN :
- 14714159 and 00223042
- Volume :
- 151
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry
- Accession number :
- edsair.doi.dedup.....5d9f3e848442f9a949b27ae006fc6ce9