Back to Search
Start Over
Single-cell transcriptional profiling identifies a cluster of potential metastasis-associated UBE2C+ cells in immature ovarian teratoma
- Source :
- Biochemical and biophysical research communications. 528(3)
- Publication Year :
- 2020
-
Abstract
- To dissect the disease heterogeneity and identify the underlying cellular and molecular events related to metastasis of immature ovarian teratoma in children, single-cell RNA sequencing was performed for a 2-year-old patient with liver metastases from immature ovarian teratoma. A total of 5976 cells were obtained for further analysis, with a median unique molecular identifier count of 6011 per cell and a median number of 1741 genes detected per cell. Fourteen clusters were recognized, with the main lineages comprising epithelial cells, macrophages, fibroblasts, glial cells, and dendritic cells. Ten subclusters of epithelial cells were further defined, originating from the urinary tract, esophagus, bronchus, lung, skin, and gastrointestinal tract. An undefined UBE2C + population in an active state of proliferation was also identified and its biological processes were related to meiosis and maturation of oocytes. Pseudotime analysis revealed different distributions of epithelial cells in the development trajectory. In conclusion, a cluster of UBE2C + epithelial cells in an active state of proliferation was identified in an immature ovarian teratoma in a child, and may contribute to metastasis by regulating epithelial–mesenchymal transition. These findings help toward understanding the origin of the malignant behaviors, offer a potential biomarker for early determination of the tumor nature, and provide new ideas for the therapy of immature ovarian teratoma in children.
- Subjects :
- 0301 basic medicine
endocrine system diseases
Cell
Population
Biophysics
Immature Ovarian Teratoma
Biology
Biochemistry
Metastasis
03 medical and health sciences
0302 clinical medicine
medicine
Biomarkers, Tumor
Humans
Cell Lineage
Epithelial–mesenchymal transition
education
Molecular Biology
Cell Proliferation
Ovarian Neoplasms
Bronchus
education.field_of_study
Gastrointestinal tract
Lung
Gene Expression Profiling
Liver Neoplasms
Teratoma
Cell Differentiation
Epithelial Cells
Cell Biology
medicine.disease
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Child, Preschool
Ubiquitin-Conjugating Enzymes
Cancer research
Female
Single-Cell Analysis
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 528
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....5d931ede9e9cd1458530358a49556799