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Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings
- Source :
- Journal of Medical Imaging, Journal of medical imaging 7(01), 013501 (1-13) (2020). doi:10.1117/1.JMI.7.1.013501
- Publication Year :
- 2020
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2020.
-
Abstract
- X-ray cone-beam holotomography of unstained tissue from the human central nervous system reveals details down to subcellular length scales. This visualization of variations in the electron density of the sample is based on phase-contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone-beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength-independent electron density. We present the multi-E holotomography at the Göttingen Instrument for Nano-Imaging with X-Rays (GINIX) setup of the P10 beamline at Deutsches Elektronen-Synchrotron. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial filtering, and high numerical aperture. Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue. We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a double-crystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed.
- Subjects :
- Diffraction
Paper
Holography
Image processing
Phase problem
tomography
030218 nuclear medicine & medical imaging
law.invention
contrast transfer function
03 medical and health sciences
0302 clinical medicine
Optics
law
Medicine
Radiology, Nuclear Medicine and imaging
ddc:610
Physics of Medical Imaging
x-ray phase-contrast microscopy
phase retrieval
Contrast transfer function
Spatial filter
business.industry
3. Good health
virtual histology
Cardinal point
030220 oncology & carcinogenesis
holography
business
Phase retrieval
Subjects
Details
- Language :
- English
- ISSN :
- 23294310 and 23294302
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Imaging
- Accession number :
- edsair.doi.dedup.....5d77ef06c83f7d72873e60ac7b2af908