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Widespread, hypoxia‐inducible expression of HIF‐2α in distinct cell populations of different organs
Widespread, hypoxia‐inducible expression of HIF‐2α in distinct cell populations of different organs
- Source :
- The FASEB Journal. 17:271-273
- Publication Year :
- 2002
- Publisher :
- Wiley, 2002.
-
Abstract
- Cellular responses to oxygen are increasingly recognized as critical in normal development and physiology, and are implicated in pathological processes. Many of these responses are mediated by the transcription factors HIF-1 and HIF-2. Their regulation occurs through oxygen-dependent proteolysis of the alpha subunits HIF-1alpha and HIF-2alpha, respectively. Both are stabilized in cell lines exposed to hypoxia, and recently HIF-1alpha was reported to be widely expressed in vivo. In contrast, regulation and sites of HIF-2alpha expression in vivo are unknown, although a specific role in endothelium was suggested. We therefore analyzed HIF-2alpha expression in control and hypoxic rats. Although HIF-2alpha was not detectable under baseline conditions, marked hypoxic induction occurred in all organs investigated, including brain, heart, lung, kidney, liver, pancreas, and intestine. Time course and amplitude of induction varied between organs. Immunohistochemistry revealed nuclear accumulation in distinct cell populations of each tissue, which were exclusively non-parenchymal in some organs (kidney, pancreas, and brain), predominantly parenchymal in others (liver and intestine) or equally distributed (myocardium). These data indicate that HIF-2 plays an important role in the transcriptional response to hypoxia in vivo, which is not confined to the vasculature and is complementary to rather than redundant with HIF-1.
- Subjects :
- Telencephalon
Pathology
medicine.medical_specialty
Time Factors
Endothelium
Duodenum
Immunoblotting
Cell
Biology
Kidney
Biochemistry
In vivo
Gene expression
Parenchyma
Basic Helix-Loop-Helix Transcription Factors
Genetics
medicine
Animals
RNA, Messenger
Hypoxia
Lung
Molecular Biology
Transcription factor
Regulation of gene expression
Myocardium
Rats
Cell biology
medicine.anatomical_structure
Gene Expression Regulation
Liver
Transcription Factors
Biotechnology
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi.dedup.....5d607ce589c8a09fb0babae2e95bc886
- Full Text :
- https://doi.org/10.1096/fj.02-0445fje