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Apoptosis in dilated cardiomyopathy
- Source :
- The Korean Journal of Internal Medicine
- Publication Year :
- 2000
-
Abstract
- Objective Cardiomyopathy, a popular diagnosis that always obscures more than it reveals, nevertheless has several characteristic histological features. These prominently include widespread focal myocardial fibrosis and associated hypertrophy of surviving cardiac myocyte. In fact, focal noninflammatory degeneration (not necrosis) has been demonstrated as a feature of many forms of cardiac hypertrophy. We hypothesized that this loss of myocardial cells in dilated cardiomyopathy (DCMP) may result from cell death by apoptosis. Methods Endomyocardial biopsy specimens from the right ventricles of six patients who suffered from DCMP were studied, and myocardial specimens from two persons who died in motor vehicle accidents were used as negative controls. For identification of apoptosis, immunohistochemistry with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling was performed. In addition, apoptosis was confirmed morphologically by confocal laser scanning microscopy with propidium iodide. Results Apoptosis, that was represented by an apoptotic index ranging from 19.8 to 25.4%, could be extensively seen in myocytes and also rarely in non-myocytes of interstitium and vascular endothelium. Morphologically, there were a lot of nuclei with clumps of condensed chromatin, suggestive of apoptosis. Conclusion The present study demonstrated that myocyte loss in DCMP might be mainly due to the apoptosis of myocytes and interstitial cells, rather than inflammation or cell necrosis.
- Subjects :
- Adult
Cardiomyopathy, Dilated
Male
Programmed cell death
Pathology
medicine.medical_specialty
Necrosis
Cardiomyopathy
Dilated cardiomyopathy
Apoptosis
Statistics, Nonparametric
chemistry.chemical_compound
Reference Values
medicine
In Situ Nick-End Labeling
Humans
Confocal laser scanning microscopy
Propidium iodide
Analysis of Variance
Enzyme Precursors
Microscopy, Confocal
business.industry
Caspase 3
Myocardium
Cardiac myocyte
Biopsy, Needle
Articles
Middle Aged
medicine.disease
Immunohistochemistry
chemistry
Terminal deoxynucleotidyl transferase
Caspases
Terminal deoxynucleotidyl transferase nick end-labeling
Myocardial fibrosis
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 12263303
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Korean journal of internal medicine
- Accession number :
- edsair.doi.dedup.....5d4ff2cd53cdaae37536a8aa8fee6eaa