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A novel polysaccharide with antioxidant, HIV protease inhibiting and HIV integrase inhibiting activities from Fomitiporia punctata (P. karst.) murrill (Basidiomycota, hymenochaetales)
- Source :
- International journal of biological macromolecules. 97
- Publication Year :
- 2016
-
Abstract
- A novel polysaccharide fraction (G1) was obtained from the fungus Fomitiporia punctata (P. Karst.) Murrill. G1 exhibited a molecular weight of approximately 151 kDa. The FT-IR results suggested that the monosaccharide components of G1 possessed furanoid rings and there were β-glycosidic bonds between the sugar units. The 1H NMR results showed that G1 was composed of arabinose, fructose, galactose and glucose in the molar ratio of 1.6:3.8:19.7:19.7, as determined by gas chromatography-mass spectrometry (GC–MS) and high performance liquid chromatography (HPLC). G1 produced significant antioxidant effects as evidenced by its potency in inhibiting erythrocyte hemolysis, and in scavenging hydroxyl radicals and superoxide radicals. The highest rates of inhibition achieved were 73.58%, 36.55% and 50.98% respectively. In addition, G1 brought about 19.6% inhibition of HIV-1 protease activity at the concentration of 50 μg/mL. G1 displayed inhibitory activity toward HIV-1 integrase in the concentration range of 100–1000 μg/mL. The present study indicates that G1 from Fomitiporia punctate (P. Karst.) Murrill is a novel natural antioxidant.
- Subjects :
- 0301 basic medicine
Arabinose
Antioxidant
Stereochemistry
medicine.medical_treatment
02 engineering and technology
HIV Integrase
Polysaccharide
Biochemistry
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
HIV Protease
Structural Biology
medicine
Monosaccharide
HIV Integrase Inhibitors
Molecular Biology
chemistry.chemical_classification
Protease
Fomitiporia
biology
Basidiomycota
Fructose
Fungal Polysaccharides
General Medicine
HIV Protease Inhibitors
021001 nanoscience & nanotechnology
biology.organism_classification
030104 developmental biology
chemistry
Galactose
0210 nano-technology
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....5d3480ea468c6b9ad6cada7705f1584b