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Dynamic Reorganization of Extremely Long-Range Promoter-Promoter Interactions between Two States of Pluripotency
- Source :
- Cell Stem Cell, 17, 6, pp. 748-757, Cell Stem Cell, 17, 748-757
- Publisher :
- Elsevier Inc.
-
Abstract
- SummarySerum-to-2i interconversion of mouse embryonic stem cells (mESCs) is a valuable in vitro model for early embryonic development. To assess whether 3D chromatin organization changes during this transition, we established Capture Hi-C with target-sequence enrichment of DNase I hypersensitive sites. We detected extremely long-range intra- and inter-chromosomal interactions between a small subset of H3K27me3 marked bivalent promoters involving the Hox clusters in serum-grown cells. Notably, these promoter-mediated interactions are not present in 2i ground-state pluripotent mESCs but appear upon their further development into primed-like serum mESCs. Reverting serum mESCs to ground-state 2i mESCs removes these promoter-promoter interactions in a spatiotemporal manner. H3K27me3, which is largely absent at bivalent promoters in ground-state 2i mESCs, is necessary, but not sufficient, to establish these interactions, as confirmed by Capture Hi-C on Eed−/− serum mESCs. Our results implicate H3K27me3 and PRC2 as critical players in chromatin alteration during priming of ESCs for differentiation.
- Subjects :
- Male
Pluripotent Stem Cells
Cellular differentiation
Mice, Transgenic
Histones
Mice
Protein Domains
Genetics
Animals
Deoxyribonuclease I
Promoter Regions, Genetic
Induced pluripotent stem cell
Hox gene
Molecular Biology
Embryonic Stem Cells
Cell Proliferation
Cell Nucleus
Homeodomain Proteins
biology
Genes, Homeobox
Cell Differentiation
Cell Biology
Embryonic stem cell
Chromatin
Cell biology
Histone
biology.protein
Molecular Medicine
PRC2
Subjects
Details
- Language :
- English
- ISSN :
- 19345909
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cell Stem Cell
- Accession number :
- edsair.doi.dedup.....5d1e8b9c7b332bc30d40a9e472566cc5
- Full Text :
- https://doi.org/10.1016/j.stem.2015.11.010