Effect of Nitrogen Concentration on the Biosynthesis of Citric Acid, Protein, and Lipids in the Yeast Yarrowia lipolytica

iYarrowia lipolytica/iyeast is well known to be able to synthesize citric acid (CA) in large amounts. This study deals with CA biosynthesis, the production of biomass, as well as the accumulation and composition of proteins and lipids iniY. lipolytica/iVKM Y-2373 grown in media with glucose at different concentrations of ammonium sulfate (from 2 to 10 g/L). It was found that these concentrations of nitrogen source are limiting for the growth ofiY. lipolytica/iand that nitrogen deficiency is the main cause of CA excretion. At the high concentration of (NHsub4/sub)sub2/subSOsub4/sub(10 g/L), the accumulation of cell biomass, biomass yield (YsubX/S/sub), and protein concentration was higher than in the medium with 2 g/L ammonium sulfate by 4.3 times, 143%, and 5.1 times, respectively. CA was accumulated in meaningful quantities only in media containing 3-10 g/L (NHsub4/sub)sub2/subSOsub4/subwith the maximum concentration of CA (99.9 g/L) at 4 g/L ammonium sulfate. Also of interest is the technological mode with 6 g/L (NHsub4/sub)sub2/subSOsub4/sub, which is characterized by high productivity (1.11 g/L × h). It should be noted that biomass contains large amounts of essential amino acids and unsaturated fatty acids and can be used in food biotechnologies and agriculture.