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Comparison of individual hive and apiary-level sample types for spores of Paenibacillus larvae in Saskatchewan honey bee operations
- Source :
- PLoS ONE, Vol 17, Iss 2, p e0263602 (2022), PLoS ONE
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Three commercial honey bee operations in Saskatchewan, Canada, with outbreaks of American foulbrood (AFB) and recent or ongoing metaphylactic antibiotic use were intensively sampled to detect spores of Paenibacillus larvae during the summer of 2019. Here, we compared spore concentrations in different sample types within individual hives, assessed the surrogacy potential of honey collected from honey supers in place of brood chamber honey or adult bees within hives, and evaluated the ability of pooled, extracted honey to predict the degree of spore contamination identified through individual hive testing. Samples of honey and bees from hives within apiaries with a recent, confirmed case of AFB in a single hive (index apiaries) and apiaries without clinical evidence of AFB (unaffected apiaries), as well as pooled, apiary-level honey samples from end-of-season extraction, were collected and cultured to detect and enumerate spores. Only a few hives were heavily contaminated by spores in any given apiary. All operations were different from one another with regard to both the overall degree of spore contamination across apiaries and the distribution of spores between index apiaries and unaffected apiaries. Within operations, individual hive spore concentrations in unaffected apiaries were significantly different from index apiaries in the brood chamber (BC) honey, honey super (HS) honey, and BC bees of one of three operations. Across all operations, BC honey was best for discriminating index apiaries from unaffected apiaries (p = 0.001), followed by HS honey (p = 0.06), and BC bees (p = 0.398). HS honey positively correlated with both BC honey (rs = 0.76, p < 0.0001) and bees (rs = 0.50, p < 0.0001) and may be useful as a surrogate for either. Spore concentrations in pooled, extracted honey seem to have predictive potential for overall spore contamination within each operation and may have prognostic value in assessing the risk of future AFB outbreaks at the apiary (or operation) level.
- Subjects :
- Life Cycles
Epidemiology
Animal Diseases
Disease Outbreaks
Larvae
Animal Products
Antibiotics
Microbial Physiology
Medicine and Health Sciences
Bacterial Physiology
Spores, Bacterial
Multidisciplinary
Antimicrobials
digestive, oral, and skin physiology
Eukaryota
Drugs
food and beverages
Agriculture
Honey
Bees
Saskatchewan
Anti-Bacterial Agents
Insects
behavior and behavior mechanisms
Medicine
Seasons
Honey Bees
Research Article
animal structures
Arthropoda
Science
Microbiology
Microbial Control
Animals
Bacterial Spores
Gram-Positive Bacterial Infections
Nutrition
Pharmacology
Colony Collapse
Paenibacillus larvae
fungi
Organisms
Biology and Life Sciences
Bacteriology
Hymenoptera
Invertebrates
Diet
Food
Medical Risk Factors
Beekeeping
Zoology
Entomology
Food Analysis
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....5cf92b049d07feb6ae0550f3e261494f