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Genomic analysis of phylogenetic group B2 extraintestinal pathogenic E. coli causing infections in dogs in Australia
- Source :
- Veterinary microbiology. 248
- Publication Year :
- 2020
-
Abstract
- This study investigated the prevalence of extraintestinal pathogenic E. coli (ExPEC)-associated sequence types (STs) from phylogenetic group B2 among 449 fluoroquinolone-susceptible dog clinical isolates from Australia. Isolates underwent PCR-based phylotyping and random amplified polymorphic DNA analysis to determine clonal relatedness. Of the 317 so-identified group B2 isolates, 77 underwent whole genome sequencing (WGS), whereas the remainder underwent PCR-based screening for ST complexes (STc) STc12, STc73, STc372, and ST131. The predominant ST was ST372 according to both WGS (31 % of 77) and ST-specific PCR (22 % of 240), followed by (per WGS) ST73 (17 %), ST12 (7 %), and ST80 (7 %). A WGS-based phylogenetic comparison of ST73 isolates from dogs, cats, and humans showed considerable overall phylogenetic diversity. Although most clusters were species-specific, some contained closely related human and animal (dog > cat) isolates. For dogs in Australia these findings both confirm ST372 as the predominant E. coli clonal lineage causing extraintestinal infections and clarify the importance of human-associated group B2 lineage ST73 as a cause of UTI, with some strains possibly being capable of bi-directional (i.e., dog-human and human-dog) transmission.
- Subjects :
- Extraintestinal Pathogenic Escherichia coli
Virulence Factors
Lineage (evolution)
Genomics
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
Polymorphism, Single Nucleotide
Host Specificity
03 medical and health sciences
Dogs
medicine
Animals
Humans
Dog Diseases
Escherichia coli
Escherichia coli Infections
Phylogeny
030304 developmental biology
Whole genome sequencing
0303 health sciences
General Veterinary
Phylogenetic tree
Virulence
Whole Genome Sequencing
030306 microbiology
Extraintestinal Pathogenic E. coli
Australia
General Medicine
Sequence types
Phylogenetic diversity
Cats
Genome, Bacterial
Subjects
Details
- ISSN :
- 18732542
- Volume :
- 248
- Database :
- OpenAIRE
- Journal :
- Veterinary microbiology
- Accession number :
- edsair.doi.dedup.....5cf7bb196776d3c987bdb025c9e8e313