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A tractable, simplified ex vivo human skin model of wound infection
- Source :
- Wound Repair Regen, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, vol 27, iss 4
- Publication Year :
- 2018
-
Abstract
- The prevalence of infection in chronic wounds is well documented in the literature, but not optimally studied due to the drawbacks of current methodologies. Here, we describe a tractable and simplified ex vivo human skin model of infection that addresses the critical drawbacks of high costs and limited translatability. Wounds were generated from excised abdominal skin from cosmetic procedures and cultured, inoculated with Staphylococcus aureus strain UAMS-1, or under aseptic conditions. After three days, the infected wounds exhibited biofilm formation and significantly impaired re-epithelialization compared to the control. Additionally, pro-migratory and pro-reparative genes were significantly downregulated while pro-inflammatory genes were significantly upregulated, demonstrating molecular characterizations of impaired healing as in chronic wounds. This model allows for a simplified and versatile tool for the study of wound infection and subsequent development of novel therapies.
- Subjects :
- Staphylococcus aureus
Cells
Clinical Sciences
Human skin
Dermatology
medicine.disease_cause
Models, Biological
Article
Proinflammatory cytokine
Tissue Culture Techniques
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Re-Epithelialization
Models
Medicine
Humans
Cosmetic procedures
Cells, Cultured
Skin
Wound Healing
Cultured
integumentary system
business.industry
Abdominal skin
Dermatology & Venereal Diseases
Staphylococcal Infections
Biological
Wound infection
Emerging Infectious Diseases
Infectious Diseases
Biofilms
Immunology
Wound Infection
Surgery
business
Infection
Ex vivo
Subjects
Details
- ISSN :
- 1524475X
- Volume :
- 27
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
- Accession number :
- edsair.doi.dedup.....5ce259115893e34afd91ed771463d46c