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Quantitation of phosphatidylserine-exposing platelets in platelet concentrate prepared in routine blood transfusion laboratory
- Source :
- Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis. 59(1)
- Publication Year :
- 2019
-
Abstract
- Background Phosphatidylserine (PS) plays important roles in platelets’ pro-coagulant function. However, little is known about assessing this molecule in platelet concentrates (PCs) prepared for routine blood transfusion service. Aim To quantitate the number of PS-exposing platelets in PCs prepared in a routine transfusion laboratory. Methods PC products were prepared according to routine laboratory procedure. The numbers of PS-exposing platelets in the PCs and in unprocessed whole blood were determined using flow cytometry. Results A cross-sectional study of 253 PCs found that they had significantly increased numbers of PS-exposing platelets compared to unprocessed whole blood (47,439 ± 26,500 cells/μL; 5903‒166,156 cells/μL) vs. 30,058 ± 12,958 cells/μL; 8,154-86,606 cells/μL). A heterogeneity study demonstrated that 6% and 2% of the measured PCs and of unprocessed donor whole blood, respectively, showed an increase in the number of PS-exposing platelets that was greater than 2 fold. Conclusions The study suggested that the number PS-exposing platelets in PC prepared in a routine transfusion laboratory differs. However, assessment of the number of PS-exposing platelets in platelet products could be a valid measure to use in managing the quality of platelet processing in routine laboratories.
- Subjects :
- Blood Platelets
Blood transfusion
medicine.medical_treatment
Phosphatidylserines
030204 cardiovascular system & hematology
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Medicine
Humans
Platelet
Blood Transfusion
Platelet concentrate
Whole blood
Chromatography
medicine.diagnostic_test
business.industry
Routine laboratory
Hematology
Phosphatidylserine
Cross-Sectional Studies
chemistry
business
Laboratories
030215 immunology
Subjects
Details
- ISSN :
- 14730502
- Volume :
- 59
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
- Accession number :
- edsair.doi.dedup.....5ce221bbbe2fdde622e173c38952c367