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IL-33 promotes airway remodeling and is a marker of asthma disease severity
- Source :
- The Journal of asthma : official journal of the Association for the Care of Asthma. 51(8)
- Publication Year :
- 2014
-
Abstract
- To investigate the function of interleukin-33 (IL-33) in the asthmatic airway remodeling and the relationship between IL-33 and asthma severity.IL-33 levels, sputum eosinophils percentage (EOS%), pulmonary function and total immunoglobulin (IgE) were measured for 45 patients with asthma and 40 non-allergic controls. Asthma severity was assessed. The expressions of IL-33 and reticular basement membrane (RBM) on bronchial biopsy specimens from eight asthma patients and eight non-allergic controls were observed after hematoxylin-eosin staining (HE) and immunohistochemical staining. In vitro experiments, real-time polymerase chain reactions and western blotting analysis were used to identify the specific effects of IL-33 administration.Serum IL-33 levels in patients with asthma were higher than those in non-allergic controls. Moreover, in asthmatic patients, serum IL-33 levels were negatively correlated to forced expiratory volume in one second (FEV1, % predicted), and positively correlated to asthma severity. Increased expression of IL-33 and RBM thickening were observed on bronchial biopsy specimens obtained from patients with asthma. Serum IL-33 levels were positively correlated to basement membrane thickness. The production of fibronectin1 and type I collagen in human lung fibroblasts (HLF-1) increased at 24 h after IL-33 treatment in vitro. Pre-treatment with anti-ST2 antibody or fluticasone propionate (FP) suppressed the production of fibronectin1 and types I collagen induced by IL-33.IL-33 is a marker of asthma severity, and may contribute to airway remodeling in asthma by acting on human lung fibroblasts.
- Subjects :
- Pulmonary and Respiratory Medicine
Adult
Male
Immunoglobulin E
Severity of Illness Index
Pulmonary function testing
Immunology and Allergy
Medicine
Bronchial Biopsy
Humans
Asthma
biology
business.industry
Interleukins
medicine.disease
Interleukin-33
respiratory tract diseases
Interleukin 33
Asthmatic Airway Remodeling
Pediatrics, Perinatology and Child Health
Immunology
biology.protein
Sputum
Airway Remodeling
Female
medicine.symptom
Airway
business
Biomarkers
Subjects
Details
- ISSN :
- 15324303
- Volume :
- 51
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The Journal of asthma : official journal of the Association for the Care of Asthma
- Accession number :
- edsair.doi.dedup.....5ccbe87601d78782c231c997d918562c