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Antibodies to B7.1 define the GFCC′C″ face of the N-terminal domain as critical for co-stimulatory interactions
- Source :
- Immunology Letters. 83:77-83
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- Antagonists of the B7 family of co-stimulatory molecules have the potential for altering immune responses therapeutically. To better define the requirements for such inhibitors, we have mapped the binding of an entire panel of blocking antibodies specific for human B7.1. By mutagenesis, each of the residues critical for blocking antibody binding appeared to fall entirely within the N-terminal V-set domain of B7.1. Thus, although antibody-antigen interacting surfaces can be quite large, these results indicate that a relatively small portion of the GFCC'C" face of this domain is crucial for further antagonist development.
- Subjects :
- Recombinant Fusion Proteins
Molecular Sequence Data
Immunology
medicine.disease_cause
Mice
Co-stimulation
Blocking antibody
medicine
Animals
Humans
Immunology and Allergy
Amino Acid Sequence
Antibodies, Blocking
Peptide sequence
Mutation
COS cells
Chemistry
Mutagenesis
Immunoglobulin Fc Fragments
Cell biology
Epitope mapping
Biochemistry
COS Cells
B7-1 Antigen
Mutagenesis, Site-Directed
Epitopes, B-Lymphocyte
Epitope Mapping
Subjects
Details
- ISSN :
- 01652478
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Immunology Letters
- Accession number :
- edsair.doi.dedup.....5ccacd896e3ee025f37ab87e169932d8