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A Transcriptomic Analysis of Gene Expression in Chieh-Qua in Response to Fusaric Acid Stress

Authors :
Piaoyun Sun
Qingwu Peng
Jinqiang Yan
Min Wang
Xiaoming He
Biao Jiang
Lin Yu'e
Feng Chen
Dasen Xie
Wenrui Liu
Source :
Horticulturae, Volume 7, Issue 4, Horticulturae, Vol 7, Iss 88, p 88 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Fusarium wilt results in undesirable effects on the quality and production of chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). Fusaric acid (FA), a secondary metabolite of biotin produced by pathogens of genus Fusarium, induced resistant responses in chieh-qua<br />however, the physiological and molecular mechanism(s) of FA resistance remains largely unknown. In our study, ‘A39’ (FA-resistant cultivar) exhibited decreased malondialdehyde (MDA) content and increased superoxide dismutase (SOD) enzyme activity when exposed to FA compared with ‘H5’ (FA-susceptible cultivar). More apoptosis cells existed in ‘H5’ than ‘A39’ after 2 days of FA treatment. RNA-seq results revealed that a total of 2968 and 3931 differentially expressed genes (DEGs) were detected under normal conditions (1562 up-regulated and 1406 down-regulated) and FA treatment (2243 up-regulated and 1688 down-regulated), respectively. Interestingly, DEGs associated with pathogen-related protein and ethylene (ET) biosynthesis and signal pathways were most significantly changed during FA stress. Notably, several crucial genes encoding pathogenesis-related protein (CL4451.Contig2, CL2175.Contig4), peroxidase (Unigene49615 and CL11695.Contig2), and ET-responsive transcription factors (TFs) (CL9320.Contig1, CL9849.Contig3, CL6826.Contig2, CL919. Contig6, and CL518.Contig7) were specifically induced after FA treatment. Collectively, the study provides molecular data for isolating candidate genes involved in FA resistance, especially ET related genes in chieh-qua.

Details

ISSN :
23117524
Volume :
7
Database :
OpenAIRE
Journal :
Horticulturae
Accession number :
edsair.doi.dedup.....5cc767200f3f4f2b1c0bdf537eeefc1d
Full Text :
https://doi.org/10.3390/horticulturae7040088