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A Transcriptomic Analysis of Gene Expression in Chieh-Qua in Response to Fusaric Acid Stress
- Source :
- Horticulturae, Volume 7, Issue 4, Horticulturae, Vol 7, Iss 88, p 88 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Fusarium wilt results in undesirable effects on the quality and production of chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). Fusaric acid (FA), a secondary metabolite of biotin produced by pathogens of genus Fusarium, induced resistant responses in chieh-qua<br />however, the physiological and molecular mechanism(s) of FA resistance remains largely unknown. In our study, ‘A39’ (FA-resistant cultivar) exhibited decreased malondialdehyde (MDA) content and increased superoxide dismutase (SOD) enzyme activity when exposed to FA compared with ‘H5’ (FA-susceptible cultivar). More apoptosis cells existed in ‘H5’ than ‘A39’ after 2 days of FA treatment. RNA-seq results revealed that a total of 2968 and 3931 differentially expressed genes (DEGs) were detected under normal conditions (1562 up-regulated and 1406 down-regulated) and FA treatment (2243 up-regulated and 1688 down-regulated), respectively. Interestingly, DEGs associated with pathogen-related protein and ethylene (ET) biosynthesis and signal pathways were most significantly changed during FA stress. Notably, several crucial genes encoding pathogenesis-related protein (CL4451.Contig2, CL2175.Contig4), peroxidase (Unigene49615 and CL11695.Contig2), and ET-responsive transcription factors (TFs) (CL9320.Contig1, CL9849.Contig3, CL6826.Contig2, CL919. Contig6, and CL518.Contig7) were specifically induced after FA treatment. Collectively, the study provides molecular data for isolating candidate genes involved in FA resistance, especially ET related genes in chieh-qua.
- Subjects :
- 0106 biological sciences
Plant Science
Horticulture
Biology
01 natural sciences
SB1-1110
Transcriptome
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Gene expression
RNA-Seq
Gene
030304 developmental biology
0303 health sciences
FA stress
DEGs
Plant culture
food and beverages
Malondialdehyde
Molecular biology
Enzyme assay
Fusarium wilt
chieh-qua
chemistry
biology.protein
Fusaric acid
WRKYs
010606 plant biology & botany
Subjects
Details
- ISSN :
- 23117524
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Horticulturae
- Accession number :
- edsair.doi.dedup.....5cc767200f3f4f2b1c0bdf537eeefc1d
- Full Text :
- https://doi.org/10.3390/horticulturae7040088