Quantitation of the hepatitis-associated antigen by electroimmunodiffusion

Electroimmunodiffusion (EID) was adapted for the detection of hepatitis-associated antigen (HAA) and compared with immunoelectroosmophoresis (IEOP) and double diffusion (DD) with respect to sensitivity and applicability to rapid screening of large numbers of sera. EID is as sensitive as IEOP, requires less antiserum, and is quantitative. Results may be obtained within 45 minutes if high-titer antiserum is available. Using this technique, 26 HAA-positive sera were identified among 10,303 potential blood donors during a 3 month period.