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Immunization with outer membrane proteins (OprF and OprI) and flagellin B protects mice from pulmonary infection with mucoid and nonmucoid Pseudomonas aeruginosa
- Source :
- Journal of Microbiology, Immunology and Infection, Vol 51, Iss 3, Pp 312-320 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Background Pseudomonas aeruginosa is a Gram-negative opportunistic bacterium, which considered as a common cause of nosocomial infection and life-threatening complications in immunocompromized and cystic fibrosis patients. Here, we evaluate the protective effect of recombinant vaccines composed of outer membrane proteins OprF and OprI alone or in combination with flagellin B against mucoid and nonmucoid pseudomonas infection. Methods BALB/C mice were immunized subcutaneous using OprF and OprI with or without flagellin B and antibody titers were determined. Serum bactericidal and opsonophagocytosis activities of immunized and control sera were estimated against mucoid and nonmucoid pseudomonas strains. Lung tissue sections from immunized and nonimmunized mice were analyzed and the levels of peripheral neutrophils infiltration into the lung and tissue inflammation were scored. Results Subcutaneous immunization using OprF and OprI with or without flagellin B elicited higher antibody titers against OprF, OprI, and flagellin B. The produced antibodies successfully opsonized both mucoid and nonmucoid strains with subsequent activation of the terminal pathway of complement that enhances killing of nonmucoid strains via complement-mediated lysis. Furthermore, opsonized mucoid and nonmucoid strains showed enhanced opsonophagocytosis via human peripheral neutrophils, a mechanism that kills P. aeruginosa when complement mediated lysis is not effective especially with mucoid strains. Immunized mice also showed a significant prolonged survival time, lower bacteremia, and reduced lung damage when compared with control nonimmunized mice. Conclusion Our data showed that mice immunized with OprF/OprI or OprF/OprI and flagellin B are significantly protected from infection caused by mucoid and nonmucoid strains of P. aeruginosa .
- Subjects :
- 0301 basic medicine
lcsh:QR1-502
flagellin B
medicine.disease_cause
lcsh:Microbiology
Mice
outer membrane proteins
Immunology and Allergy
nonmucoid
Cloning, Molecular
Lung
Respiratory Tract Infections
Mice, Inbred BALB C
Vaccines, Synthetic
biology
Vaccination
Pseudomonas
Antibody titer
General Medicine
Antibodies, Bacterial
Infectious Diseases
Pseudomonas aeruginosa
Female
Antibody
Bacterial outer membrane
Bacterial Outer Membrane Proteins
Microbiology (medical)
congenital, hereditary, and neonatal diseases and abnormalities
Pseudomonas Vaccines
Lipoproteins
Recombinant Fusion Proteins
030106 microbiology
mucoid
Microbiology
03 medical and health sciences
Bacterial Proteins
Phagocytosis
Pseudomonas infection
Immunology and Microbiology(all)
medicine
Animals
Pseudomonas Infections
Opsonin
General Immunology and Microbiology
Gene Expression Regulation, Bacterial
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
medicine.disease
digestive system diseases
respiratory tract diseases
Disease Models, Animal
030104 developmental biology
biology.protein
Immunization
Flagellin
Subjects
Details
- ISSN :
- 16841182
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology, Immunology and Infection
- Accession number :
- edsair.doi.dedup.....5cbdbcaae432c9a7309dde8ebe4ebdb0