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Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets
- Source :
- Journal of Cellular and Molecular Medicine, 19(8), 1836. Wiley-Blackwell, Journal of cellular and molecular medicine, 19(8), 1836-1846. Wiley-Blackwell, Journal of Cellular and Molecular Medicine, Journal of cellular and molecular medicine, 1836-46, STARTPAGE=1836;ENDPAGE=46;TITLE=Journal of cellular and molecular medicine, Journal of Cellular and Molecular Medicine. Wiley-Blackwell
- Publication Year :
- 2015
-
Abstract
- Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100–150 μm consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (
- Subjects :
- Male
endocrine system diseases
type 1 diabetes
Cell
Mice, SCID
CULTURE
Non-U.S. Gov't
Cells, Cultured
Cell Aggregation
islets
geography.geographical_feature_category
Research Support, Non-U.S. Gov't
MIN6 PSEUDOISLETS
Middle Aged
Islet
Cell aggregation
Cell biology
medicine.anatomical_structure
METIS-310109
Molecular Medicine
PDX1
Female
EXPRESSION
medicine.medical_specialty
endocrine system
Cell Survival
Biology
Research Support
INSULIN-SECRETION
Islets of Langerhans
Cell Line, Tumor
Internal medicine
IR-95265
medicine
Journal Article
Animals
Humans
SEGREGATION
Comparative Study
pseudoislets bioengineering beta cells
Insulinoma
Cell Size
RELEASE
Type 1 diabetes
geography
TRANSPLANTATION
Pancreatic islets
islets type 1 diabetes
Reproducibility of Results
Original Articles
Cell Biology
pseudoislets bioengineering
HUMAN BETA-CELLS
medicine.disease
Transplantation
beta cells
Glucose
Endocrinology
MOLECULE N-CAM
CLUSTERS
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine, 19(8), 1836. Wiley-Blackwell, Journal of cellular and molecular medicine, 19(8), 1836-1846. Wiley-Blackwell, Journal of Cellular and Molecular Medicine, Journal of cellular and molecular medicine, 1836-46, STARTPAGE=1836;ENDPAGE=46;TITLE=Journal of cellular and molecular medicine, Journal of Cellular and Molecular Medicine. Wiley-Blackwell
- Accession number :
- edsair.doi.dedup.....5c9281d343f83276ff45369cae27ce69