Cationic porphyrin derivatives as inhibitors of polyamine catabolism

The effects of six cationic porphyrins on several enzymes involved in polyamine biosynthesis and catabolism have been examined. Both spermidine and spermine synthase were unaffected by the porphyrins at up to 2 mM. By contrast, omithine and S -adenosylmethionine decarboxylase were inhibited by the nickel and cobalt derivatives of meso -tetrakis( N -methyl-4-pyridiniumyl)porphyrin (T4MPyP) with ic 50 values in the 10–100 μM region. Spermidine/spermine N ′-acetyltransferase (SSAT) and polyamine oxidase (PAO) were highly sensitive to the six meso -substituted cationic porphyrins tested, with K i values as low as 6 nM for SSAT and 85 nM for PAO. These inhibitors may prove useful in defining the structural features of the active site of these enzymes.