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New Diagnostic Tool for Ion Channel Activity Hidden Behind the Dwell-Time Correlations

Authors :
Przemysław Borys
Paulina Trybek
Beata Dworakowska
Piotr Bednarczyk
Agata Wawrzkiewicz-Jałowiecka
Source :
The Journal of Physical Chemistry B. 126:4236-4245
Publication Year :
2022
Publisher :
American Chemical Society (ACS), 2022.

Abstract

The patch-clamp technique is a powerful tool that allows for a long observation of transport protein activity in real time. Experimental traces of single-channel currents can be considered as a record of the channel's conformational switching related to its activation and gating. In this work, we present a mathematically simple method of patch-clamp data analysis that assesses the connectivity and occupancy of distinct conformational substates of the channel. The proposed approach appears to be a big step forward due to its possible applications in the determination of channel substates related to disease and in the analysis of drug-channel interactions on the level of repetitive sequences of channel conformations. This is especially important in cases when molecular dynamics docking is impossible and Markovian modeling requires ambiguous optimization tasks.

Details

ISSN :
15205207 and 15206106
Volume :
126
Database :
OpenAIRE
Journal :
The Journal of Physical Chemistry B
Accession number :
edsair.doi.dedup.....5c7bf90fd7eaa468786c38a3db1d635c
Full Text :
https://doi.org/10.1021/acs.jpcb.2c02272