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A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae
- Source :
- Journal of bacteriology. 174(14)
- Publication Year :
- 1992
-
Abstract
- We have isolated a thiamine auxotrophic mutant carrying a recessive mutation which lacks the positive regulatory gene, THI3, which differs in the regulation of thiamine transport from the THI2 (PHO6) gene described previously (Y. Kawasaki, K. Nosaka, Y. Kaneko, H. Nishimura, and A. Iwashima, J. Bacteriol. 172:6145-6147, 1990) for expression of thiamine metabolism in Saccharomyces cerevisiae. The mutant (thi3) had a markedly reduced thiamine transport system as well as reduced activity of thiamine-repressible acid phosphatase and of several enzymes for thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-beta-hydroxyethylthiazole. These results suggest that thiamine metabolism in S. cerevisiae is subject to two positive regulatory genes, THI2 (PHO6) and THI3. We have also isolated a hybrid plasmid, pTTR1, containing a 6.2-kb DNA fragment from an S. cerevisiae genomic library which complements thiamine auxotrophy in the thi3 mutant. This gene was localized on a 3.0-kb ClaI-BglII fragment in the subclone pTTR5. Complementation of the activities for thiamine metabolism in the thi3 mutant transformed by some plasmids with the THI3 gene was also examined.
- Subjects :
- Auxotrophy
Saccharomyces cerevisiae
Mutant
Acid Phosphatase
Mutagenesis (molecular biology technique)
Biology
Microbiology
Transformation, Genetic
Genes, Regulator
Thiamine
Cloning, Molecular
Molecular Biology
Regulator gene
Thiamine transport
food and beverages
Biological Transport
biology.organism_classification
Molecular biology
Complementation
Thiazoles
Phenotype
Pyrimidines
Biochemistry
Mutagenesis
Ethyl Methanesulfonate
Enzyme Repression
human activities
Subcellular Fractions
Research Article
Subjects
Details
- ISSN :
- 00219193
- Volume :
- 174
- Issue :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of bacteriology
- Accession number :
- edsair.doi.dedup.....5c7a6052fdaef186f7f9b8ebf8c0e121