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Imaging and structural studies of DNA–protein complexes and membrane ion channels
- Source :
- Nanoscale. 9:2768-2777
- Publication Year :
- 2017
- Publisher :
- Royal Society of Chemistry (RSC), 2017.
-
Abstract
- In bio-imaging by electron microscopy, damage of the sample and limited contrast are the two main hurdles for reaching high image quality. We extend a new preparation method based on nanofabrication and super-hydrophobicity to the imaging and structural studies of nucleic acids, nucleic acid–protein complexes (DNA/Rad51 repair protein complex) and neuronal ion channels (gap-junction, K+ and GABAA channels) as paradigms of biological significance and increasing complexity. The preparation method is based on the liquid phase and is compatible with physiological conditions. Only in the very last stage, samples are dried for TEM analysis. Conventional TEM and high-resolution TEM (HRTEM) were used to achieve a resolution of 3.3 and 1.5 Å, respectively. The EM dataset quality allows the determination of relevant structural and metrological information on the DNA structure, DNA–protein interactions and ion channels, allowing the identification of specific macromolecules and their structure.
- Subjects :
- 0301 basic medicine
Materials science
Lipid Bilayers
Nanotechnology
Inbred C57BL
Electron
Ion Channels
Mice
03 medical and health sciences
Microscopy, Electron, Transmission
Nucleic Acids
Microscopy
Animals
Transmission
General Materials Science
Lipid bilayer
High-resolution transmission electron microscopy
Ion channel
Neurons
Resolution (electron density)
DNA
Mice, Inbred C57BL
030104 developmental biology
Membrane
N/A
Biophysics
Nucleic acid
Rad51 Recombinase
Macromolecule
Subjects
Details
- ISSN :
- 20403372 and 20403364
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Nanoscale
- Accession number :
- edsair.doi.dedup.....5c77f951cebed4c9bcc73a267ea94e9b