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Analysis of mRNA expression for steroidogenic enzymes in the remaining adrenal cortices attached to adrenocortical adenomas
- Source :
- European journal of endocrinology. 158(6):867-878
- Publication Year :
- 2008
- Publisher :
- European Federation of Endocrine Societies, 2008.
-
Abstract
- DESIGN AND METHODS: We have recently demonstrated that the adrenal cortices attached to aldosterone-producing adenoma (APA) contained microscopic subcapsular micronodules suggestive of active aldosterone production. In this study, we used in situ hybridization to investigate the mRNA expression of steroidogenic enzymes in the adrenal cortices attached to cortisol-producing adenoma (CPA) and clinically silent adenoma (non-functioning adenoma; NFA), in addition to APA. RESULTS: Microscopic subcapsular micronodules, which were several hundreds of micrometers in size and spheroid in shape, were observed in the cortices attached to CPA and NFA, as well as APA, at high frequency. Most of the cortical nodules in zona fasciculata to zona reticularis showed a suppressed steroidogenesis in the cortices attached to adenoma, but some expressed intensely all necessary steroidogenic enzyme mRNAs for cortisol synthesis. CONCLUSIONS: It is thus necessary to keep in mind, on the occasion of subtotal adrenalectomy, that lesions with the potential to later develop into functional adrenocortical nodules may be present in other parts of the ipsilateral or contralateral adrenal cortices.<br />European journal of endocrinology, 158(6), pp.867-878; 2008
- Subjects :
- Adult
medicine.medical_specialty
Adenoma
Endocrinology, Diabetes and Metabolism
steroidogenic enzyme
Gene Expression
In situ hybridization
Biology
Adrenocortical adenoma
chemistry.chemical_compound
Endocrinology
Zona fasciculata
Internal medicine
remaining adrenal cortex
medicine
Conn Syndrome
Humans
Cholesterol Side-Chain Cleavage Enzyme
RNA, Messenger
Aged
Aged, 80 and over
micronodule
Aldosterone
Adrenal cortex
Reverse Transcriptase Polymerase Chain Reaction
Steroid 17-alpha-Hydroxylase
General Medicine
Middle Aged
medicine.disease
Adrenal Cortex Neoplasms
DNA-Binding Proteins
adrenocortical adenoma
medicine.anatomical_structure
chemistry
Adrenal Cortex
in situ hybridization
Sulfotransferases
Zona reticularis
Subjects
Details
- Language :
- English
- ISSN :
- 1479683X
- Volume :
- 158
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- European journal of endocrinology
- Accession number :
- edsair.doi.dedup.....5c746aa58dc1898332770c88026b0914