Back to Search
Start Over
Synchrotron FTIR microspectroscopy of Escherichia coli at single-cell scale under silver-induced stress conditions
- Source :
- Analytical and Bioanalytical Chemistry, Analytical and Bioanalytical Chemistry, 2013, 405 (8), pp.2685-2697. ⟨10.1007/s00216-013-6725-4⟩, Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (8), pp.2685-2697. ⟨10.1007/s00216-013-6725-4⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- International audience; The present work was focused on elucidating biochemical changes in the model bacterium Escherichia coli exposed to ionic silver mediated stress, at a single-cell scale. In order to achieve this, in situ synchrotron Fourier-transform infrared (sFTIR) microspectroscopy was performed, for the first time, on individual cells by attenuated total reflectance (ATR) combined with the use of zinc-selenide hemisphere for high spatial resolution. In a first part, the potential of the method was evaluated on bacteria subjected to a lethal 100 μM AgNO3 concentration for 2 h compared to untreated 100 % viable cells. Differences in cell composition were assessed for the C–H stretching and protein spectral regions, indicating that the inhibitory action was targeted against both fatty acids and proteins. Transmission electron microscopy (TEM) confirmed morphological damages of the cell ultrastructure. The relevance of ATR-sFTIR microspectroscopy for highlighting the heterogeneity in Ag+-mediated effects within a given bacterial population was also pointed out. In a second part, cells were exposed to sub-lethal Ag+ concentrations (
- Subjects :
- In situ
Analytical chemistry
medicine.disease_cause
Biochemistry
Analytical Chemistry
03 medical and health sciences
Stress, Physiological
Spectroscopy, Fourier Transform Infrared
medicine
Escherichia coli
Fourier transform infrared spectroscopy
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
Protein secondary structure
030304 developmental biology
0303 health sciences
biology
Ionic silver
030306 microbiology
Chemistry
Synchrotron FTIR-ATR microspectroscopy
Single-cell scale
[CHIM.MATE]Chemical Sciences/Material chemistry
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Antibacterial effect
Transmission electron microscopy
Attenuated total reflection
Ultrastructure
Biophysics
Silver Nitrate
Single-Cell Analysis
[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Synchrotrons
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 16182642 and 16182650
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry, Analytical and Bioanalytical Chemistry, 2013, 405 (8), pp.2685-2697. ⟨10.1007/s00216-013-6725-4⟩, Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (8), pp.2685-2697. ⟨10.1007/s00216-013-6725-4⟩
- Accession number :
- edsair.doi.dedup.....5c71db6ddc5742c529c23840d1c88bbe