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Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen
- Source :
- Osteoarthritis and Cartilage. (10):1197-1207
- Publisher :
- Osteoarthritis Research Society International. Published by Elsevier Ltd.
-
Abstract
- Summary Objective Meniscal regeneration was previously shown to be enhanced by injection of mesenchymal stem/stromal cells (MSCs) but the mode of action of the MSCs was not established. The aim of this study was to define how injection of MSCs enhances meniscal regeneration. Design A hemi-meniscectomy model in rats was used. Rat-MSCs (rMSCs) or human-MSCs (hMSCs) were injected into the right knee joint after the surgery, and PBS was injected into the left. The groups were compared macroscopically and histologically at 2, 4, and 8 weeks. The changes in transcription in both human and rat genes were assayed by species-specific microarrays and real-time RT-PCRs. Results Although the number of hMSCs decreased with time, hMSCs enhanced meniscal regeneration in a manner similar to rMSCs. hMSCs injection increased expression of rat type II collagen (rat-Col II), and inhibited osteoarthritis progression. The small fraction of hMSCs was activated to express high levels of a series of genes including Indian hedgehog (Ihh), parathyroid hormone-like hormone (PTHLH), and bone morphogenetic protein 2 (BMP2). The presence of hMSCs triggered the subsequent expression of rat-Col II. An antagonist of hedgehog signaling inhibited the expression of rat-Col II and an agonist increased expression of rat-Col II in the absence of hMSCs. Conclusions Despite rapid reduction in cell numbers, intra-articular injected hMSCs were activated to express Ihh, PTHLH, and BMP2 and contributed to meniscal regeneration. The hedgehog signaling was essential in enhancing the expression of rat-Col II, but several other factors provided by the hMSCs probably contributed to the repair.
- Subjects :
- Male
endocrine system
Stromal cell
Indian hedgehog
Cell Transplantation
Biomedical Engineering
Type II collagen
Gene Expression
Cell Count
MSCs
Bone morphogenetic protein 2
Osteoarthritis
Mesenchymal Stem Cell Transplantation
Menisci, Tibial
Injections, Intra-Articular
Rheumatology
Gene expression
medicine
Animals
Humans
Regeneration
Hedgehog Proteins
Orthopedics and Sports Medicine
Meniscus
Collagen Type II
biology
Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
medicine.disease
biology.organism_classification
equipment and supplies
Hedgehog signaling pathway
Rats
Cell biology
Disease Models, Animal
Rats, Inbred Lew
Immunology
Parathyroid hormone-like hormone
Subjects
Details
- Language :
- English
- ISSN :
- 10634584
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Osteoarthritis and Cartilage
- Accession number :
- edsair.doi.dedup.....5c51070ec02b3dd8003db76b39087a6b
- Full Text :
- https://doi.org/10.1016/j.joca.2012.06.002