Back to Search Start Over

Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Authors :
Alexander Marchanka
Teresa Carlomagno
Guido Pintacuda
Jan Stanek
Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Centre for Biomolecular Drug Research (BMWZ) and Institute of Organic Chemistry
Leibniz Universität Hannover [Hannover] (LUH)
Biological Solid-State NMR Methods - Méthodes de RMN à l'état solide en biologie
Institut des Sciences Analytiques (ISA)
Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
Helmholtz Centre for Infection Research
Source :
Chemical Communications, Chemical communications (Cambridge, England), Chemical Communications, Royal Society of Chemistry, 2018, 54 (65), pp.8972-8975. ⟨10.1039/c8cc04437f⟩, Chemical Communications 54 (2018), Nr. 65
Publication Year :
2018

Abstract

Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow 1H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly 13C,15N-labelled RNA.

Details

ISSN :
13597345 and 1364548X
Database :
OpenAIRE
Journal :
Chemical Communications
Accession number :
edsair.doi.dedup.....5c34e72fadb5c9ea8a043c6a106eada2
Full Text :
https://doi.org/10.1039/c8cc04437f