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Early hepatic immune response in rats infected with Fasciola hepatica
- Source :
- Veterinary Research (33), 261-270. (2002)
- Publication Year :
- 2002
-
Abstract
- We investigated the phenotype of the T cells (CD4 + and CD8 + ) that produced Th1 (IFN-γ ) and Th2 cytokines (IL-4 and IL-10) during the first two weeks of experimental fasciolosis in rats. We also followed the kinetics of the cytokine and proliferative responses of hepatic mononuclear cells (HMNC) over the same period. We found that HMNC were more numerous in the infected ani- mals than in the controls. The percentage of CD4 + cells increased significantly after infection, whereas the percentage of CD8 + cells did not change. Moreover, the frequency of the cells producing (CP) cytokine changed after infection. The frequency of CP IFN-γ on 7 days postinfection (pi) was similar to that in control animals. However, the frequency of CP IFN-γ was clearly lower on day 14 pi, whereas the frequency of CP IL-4 and CP IL-10 had increased. The CP IL-10-were mostly CD4 + . Mitogenic stimulation (phorbol myristate acetate/ionomycin) of HMNC led to an increase in the amounts of the Th2 cytokines in the supernatant on days 7 and 14 pi, with the increase more pro- nounced on day 14. In contrast, IFN-γ levels also increased by day 7 pi but then decreased to below control levels by day 14. In addition, HMNC proliferation in response to mitogen followed a similar pattern to IFN-γ production. These findings suggested that, during the first 2 weeks of infection, F. hepatica induced a transient Th0 cytokine profile followed by downregulation of the cellular re- sponse and the induction of a Th2 cytokine profile. Fasciola hepatica / rat / cytokine / liver / lymphocytes
- Subjects :
- CD4-Positive T-Lymphocytes
Male
Cellular immunity
medicine.medical_treatment
Lymphocyte
CD8-Positive T-Lymphocytes
Lymphocyte Activation
rattus rattus
trematode
chemistry.chemical_compound
0302 clinical medicine
cytokine
0303 health sciences
foie
Flow Cytometry
3. Good health
Interleukin-10
Cytokine
medicine.anatomical_structure
Liver
Ionomycin
Cytokines
Veterinary medicine and animal Health
Female
Fascioliasis
lymphocyte
Biology
Peripheral blood mononuclear cell
Andrology
03 medical and health sciences
Interferon-gamma
Immune system
medicine
Animals
Rats, Wistar
030304 developmental biology
General Veterinary
Fasciola hepatica
Hepatic immune response
Rats
Kinetics
Médecine vétérinaire et santé animal
chemistry
helminthe
Immunology
Leukocytes, Mononuclear
RAT
Interleukin-4
CD8
030215 immunology
Subjects
Details
- ISSN :
- 09284249
- Volume :
- 33
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Veterinary research
- Accession number :
- edsair.doi.dedup.....5c097dcbbdcd8fe0cbff41dca4edde33