Back to Search
Start Over
Nuclear localization of the 82-kDa form of human choline acetyltransferase
- Source :
- The Journal of biological chemistry. 274(27)
- Publication Year :
- 1999
-
Abstract
- Choline acetyltransferase is the enzyme catalyzing synthesis of the neurotransmitter acetylcholine in cholinergic neurons. In human, transcripts encoding two forms of the enzyme with apparent molecular masses of 69 and 82 kDa are found in brain and spinal cord; the 82-kDa form differs from the 69-kDa enzyme only in terms of a 118-amino acid extension on its amino terminus. Using green fluorescent protein-tagged choline acetyltransferase, we show that the 82-kDa enzyme is targeted to nuclei of cells, whereas the 69-kDa protein is found in cytoplasm. Expression of site-directed and deletion mutants of the 82-kDa isoform reveals that the extended amino terminus contains a nuclear localization signal in the first nine amino acids which targets the protein to nucleus. This represents the first report of a neurotransmitter-synthesizing enzyme that is localized to the cell nucleus.
- Subjects :
- Cytoplasm
Green Fluorescent Proteins
Molecular Sequence Data
Biology
Transfection
Biochemistry
Cell Line
Choline O-Acetyltransferase
medicine
Humans
Amino Acid Sequence
Molecular Biology
Peptide sequence
Fluorescent Dyes
chemistry.chemical_classification
Cell Nucleus
Microscopy, Confocal
Cell Biology
Choline acetyltransferase
Amino acid
Molecular Weight
Cell nucleus
Luminescent Proteins
medicine.anatomical_structure
chemistry
Nucleus
Acetylcholine
Nuclear localization sequence
medicine.drug
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 274
- Issue :
- 27
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....5c0245f5b00a5b36e2bf3a5043ee9504