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A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- This study reports a fast, sensitive, and selective column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine the endocannabinoids (eCBs), anandamide (AEA), and 2-arachidonoylglycerol (2-AG) in plasma samples. This bidimensional system used a restricted access media column (RP-8 ADS, 25 mm × 4 mm × 25 μM) in the first dimension and a core-shell Kinetex C18 (100 mm × 2, 1.7 mm × 1 μM) column in the second dimension, followed by detection in a mass spectrometer triple quadrupole (multiple reactions monitoring mode) operating in the positive mode. RP-8 ADS was used for trace enrichment of eCBs (reverse phase partitioning) and macromolecular matrix size exclusion; the core-shell column was used for the chromatographic separation. The column switching UHPLC-MS/MS method presented a linear range spanning from 0.1 ng mL−1 (LOQ) to 6 ng mL−1 for AEA and from 0.04 ng mL−1 (LOQ) to 10 ng mL−1 for 2-AG. Excluding the LLOQ values, the precision assays provided coefficients of variation lower than 8% and accuracy with relative standard error values lower than 14%. Neither carryover nor matrix effects were detected. This high-throughput column switching method compared to conventional methods is time saving as it involves fewer steps, consumes less solvent, and presents lower LLOQ. The column switching UHPLC-MS/MS method was successfully applied to determine AEA and 2-AG in plasma samples obtained from Alzheimer’s disease patients.
- Subjects :
- Polyunsaturated Alkamides
CANABINOIDES
Analytical chemistry
Arachidonic Acids
Mass spectrometry
01 natural sciences
Biochemistry
Glycerides
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Alzheimer Disease
Limit of Detection
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Phase (matter)
Humans
Chromatography, High Pressure Liquid
Column (data store)
Chromatography
Chemistry
010401 analytical chemistry
Reproducibility of Results
Equipment Design
Anandamide
0104 chemical sciences
Triple quadrupole mass spectrometer
Solvent
Linear range
030217 neurology & neurosurgery
Endocannabinoids
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 409
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....5bf713511c2a00a59e6bee1fdb15b339