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Behaviour of α-aminoadipylcysteine and glutamylcysteine in the presence of intact and disrupted mycelium of a Cephalosporium sp
- Source :
- Biochemical Journal. 112:389-396
- Publication Year :
- 1969
- Publisher :
- Portland Press Ltd., 1969.
-
Abstract
- 1. delta-(l-alpha-Aminoadipyl)-l-cysteine, the corresponding d- and dl-alpha-aminoadipyl isomers, delta-(dl-alpha-amino[6-(14)C]adipyl)-l-cysteine and gamma- and alpha-l-glutamyl-l-cysteine were synthesized. 2. The behaviour of delta-(l-aminoadipyl)-l-cysteine and the corresponding d- and dl-alpha-aminoadipyl isomers was studied in the presence of suspensions of intact mycelium of a Cephalosporium sp., suspensions treated ultrasonically and extracts obtained by grinding with alumina. 3. With intact mycelium the l-alpha-aminoadipyl isomer was removed more rapidly from the extracellular fluid than the corresponding d-isomer. 4. Addition of delta-(dl-alpha-amino[6-(14)C]adipyl)-l-cysteine to suspensions of intact mycelium led to the labelling of extracellular and intracellular penicillin N and cephalosporin C, but also to extensive hydrolysis of the dipeptide. 5. Broken-cell systems hydrolysed delta-(l-alpha-aminoadipyl)-l-cysteine and the corresponding d-alpha-aminoadipyl isomer, but the former was hydrolysed more readily than the latter. 6. gamma- and alpha-l-Glutamyl-l-cysteine were also hydrolysed but delta-(l-alpha-aminoadipyl)-l-cysteinyl-l-valine was not. 7. Only part of the enzyme activity in broken-cell systems responsible for the hydrolysis of delta-(alpha-aminoadipyl)-l-cysteine was present in the supernatant obtained on centrifugation at 20000g. 8. Possible implications of these findings are discussed.
- Subjects :
- Electrophoresis
Paper
History
Stereochemistry
Adipates
Penicillins
Vibration
Education
chemistry.chemical_compound
Hydrolysis
Glutamates
Extracellular
Centrifugation
Cysteine
Amino Acids
Mycelium
Carbon Isotopes
Dipeptide
Chromatography
biology
Oxides
Stereoisomerism
Articles
Dipeptides
Cephalosporin C
Enzyme assay
Aminopterin
Cephalosporins
Computer Science Applications
Acremonium
chemistry
biology.protein
Intracellular
Subjects
Details
- ISSN :
- 03063283
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....5bf455787cb0056d849edd06fc6a87c7