Back to Search
Start Over
Mucosal and systemic immune responses to a recombinant protein expressed on the surface of the oral commensal bacterium Streptococcus gordonii after oral colonization
- Source :
- Università degli Studi di Siena-IRIS
- Publication Year :
- 1995
-
Abstract
- To circumvent the need to engineer pathogenic microorganisms as live vaccine-delivery vehicles, a system was developed which allowed for the stable expression of a wide range of protein antigens on the surface of Gram-positive commensal bacteria. The human oral commensal Streptococcus gordonii was engineered to surface express a 204-amino acid allergen from hornet venom (Ag5.2) as a fusion with the anchor region of the M6 protein of Streptococcus pyogenes. The immunogenicity of the M6-Ag5.2 fusion protein was assessed in mice inoculated orally and intranasally with a single dose of recombinant bacteria, resulting in the colonization of the oral/pharyngeal mucosa for 10-11 weeks. A significant increase of Ag5.2-specific IgA with relation to the total IgA was detected in saliva and lung lavages when compared with mice colonized with wild-type S. gordonii. A systemic IgG response to Ag5.2 was also induced after oral colonization. Thus, recombinant Gram-positive commensal bacteria may be a safe and effective way of inducing a local and systemic immune response.
- Subjects :
- Immunoglobulin A
Saliva
Recombinant Fusion Proteins
Wasps
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Immunoglobulin G
Microbiology
Mice
Immune system
Bacterial Proteins
medicine
Animals
Stomatognathic System
Arthropod Venoms
Antigens, Bacterial
Mice, Inbred BALB C
Multidisciplinary
Mucous Membrane
biology
Streptococcus
Immunogenicity
Streptococcus gordonii
Mouth Mucosa
biology.organism_classification
Antibodies, Bacterial
Immunology
Streptococcus pyogenes
biology.protein
Pharynx
Female
Carrier Proteins
Bacterial Outer Membrane Proteins
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Università degli Studi di Siena-IRIS
- Accession number :
- edsair.doi.dedup.....5bef40985ce34dd9b86113b80a8c10e1