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Transcriptomics Identify CD9 as a Marker of Murine IL-10-Competent Regulatory B Cells
- Source :
- Cell Reports, Vol 13, Iss 6, Pp 1110-1117 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- SummaryRegulatory B cells (Breg) have immune suppressive functions in various autoimmune/inflammation models and diseases and are found to be enriched in diverse B cell subsets. The lack of a unique marker or set of markers efficiently identifying Breg cells impedes detailed investigation into their origin, development, and immunological roles. Here, we perform transcriptome analysis of IL-10-expressing B cells to identify key regulators for Breg biogenesis and function and identify CD9, a tetraspanin-family transmembrane protein, as a key surface marker for most mouse IL-10+ B cells and their progenitors. CD9 plays a role in the suppressive function of IL-10+ B cells in ex vivo T cell proliferation assays through a mechanism that is dependent upon B/T cell interactions. CD9+ B cells also demonstrate inhibition of Th1-mediated contact hypersensitivity in an in vivo model system. Taken together, our findings implicate CD9 in the immunosuppressive activity of regulatory B cells.
- Subjects :
- T cell
Regulatory B cells
Inflammation
Biology
Article
Tetraspanin 29
General Biochemistry, Genetics and Molecular Biology
Transcriptome
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
medicine
Animals
Progenitor cell
lcsh:QH301-705.5
Cells, Cultured
B cell
030304 developmental biology
B-Lymphocytes, Regulatory
0303 health sciences
Interleukin-10
3. Good health
Cell biology
Mice, Inbred C57BL
Interleukin 10
medicine.anatomical_structure
lcsh:Biology (General)
030220 oncology & carcinogenesis
embryonic structures
medicine.symptom
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 13
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....5baab92d18bf5fb2f988820cf779a37f
- Full Text :
- https://doi.org/10.1016/j.celrep.2015.09.070