Supplementary Figures S1-S11 from Distinct Subpopulations of Head and Neck Cancer Cells with Different Levels of Intracellular Reactive Oxygen Species Exhibit Diverse Stemness, Proliferation, and Chemosensitivity

Supplementary Figures S1-S11. The co-expression profile among ALDH activity, memGrp78 and Glut3 in SAS Sphere cells and cisplatin-resistant (cisPtR) SAS cells was examined by FACS (S1); Single-cell suspension from parental SAS or sphere SAS cells was stained with CellROX Deep Red reagent and DCF (S2); Enrichment of the drug-resistant population after in vitro drug treatment (S3); ROSLow cells were sorted using DCFDA staining from SAS sphere cells (S4); Differentially expressed genes of reactive oxygen species scavenging in Parental cells and Sphere cells under 2, 3, 5, or 9 weeks of cultivation with defined serum-free selection medium were collected and analyzed (S5); Treatment of sphere cells with catalase inhibitor 3AT reduced catalase activity (S6); Combinatorial treatment of ROS scavenger inhibitor and cisplatin induces apoptosis cell death (S7); Knockdown of CAT or SOD2 gene expression diminished spheres-forming capability, stemness marker expression of HN-CICs (S8); Overexpression of CAT in HNCICs promotes stemness properties (S9); Overexpression of CAT in HNSCC under defined serum-free selection medium promotes stemness properties (S10); Cell viability of drugs treated hematopoietic stem cells (S11).