Back to Search
Start Over
HuC/D expression in small round cell tumors and neuroendocrine tumors: a useful tool for distinguishing neuroblastoma from childhood small round cell tumors
- Source :
- Human pathology. 85
- Publication Year :
- 2018
-
Abstract
- The RNA-binding protein HuC/D displays a neuron-specific expression and is involved in neuronal differentiation and the maintenance of the nervous system. Here we investigated the diagnostic value of HuC/D in neuroblastomas. We evaluated 85 neuroblastic tumors: 81 neuroblastomas; 3 ganglioneuroblastomas, intermixed; 1 ganglioneuroma, maturing; and 101 other tumors consisting of 34 Ewing sarcomas, 14 nephroblastomas, 11 rhabdomyosarcomas, 15 pulmonary small cell carcinomas, 18 pancreatic neuroendocrine tumors, and 9 pheochromocytomas. Immunohistochemistry for HuC/D, PHOX2B, and tyrosine hydroxylase was performed. The immunoreactivity for HuC/D was semiquantified using the total score (TS; range, 0-8). HuC/D positivity was defined as a TS ≥6. The TS of the neuroblastic tumors (mean TS, 7.94) was significantly higher than those of the other small round cell tumors and neuroendocrine tumors (P < .001) except for the pheochromocytomas (mean TS, 6.89; P = .074). HuC/D was positive in all 85 neuroblastic tumors, 1 (2.9%) Ewing sarcoma, 1 (6.7%) pulmonary small cell carcinoma, and 8 (89%) pheochromocytomas. PHOX2B was positive in all of the neuroblastic tumors and pheochromocytomas. Tyrosine hydroxylase was positive in 80 (94%) neuroblastic tumors, 1 (9.1%) rhabdomyosarcoma, and all of the pheochromocytomas. Therefore, HuC/D serves as a highly sensitive diagnostic marker to distinguish neuroblastomas from other small round cell tumors. The combination of HuC/D and PHOX2B staining may be valuable for the diagnosis of neuroblastic tumors, especially in the assessment of small sections. HuC/D expression in tumors may be related to catecholamine production or a neural crest-derived cell origin.
- Subjects :
- 0301 basic medicine
Adult
Male
Pathology
medicine.medical_specialty
Adolescent
Adrenal Gland Neoplasms
ELAV-Like Protein 3
Pheochromocytoma
Biology
Neuroendocrine tumors
Small-cell carcinoma
Sensitivity and Specificity
Pathology and Forensic Medicine
Diagnosis, Differential
03 medical and health sciences
Neuroblastoma
Young Adult
0302 clinical medicine
medicine
Humans
Ganglioneuroma
Carcinoma, Small Cell
Rhabdomyosarcoma
Child
Aged
Aged, 80 and over
Homeodomain Proteins
Ganglioneuroblastoma
Infant, Newborn
Infant
Middle Aged
medicine.disease
Neuroblastic Tumor
Pancreatic Neoplasms
Neuroendocrine Tumors
030104 developmental biology
nervous system
030220 oncology & carcinogenesis
Child, Preschool
Immunohistochemistry
Female
Sarcoma
Transcription Factors
Subjects
Details
- ISSN :
- 15328392
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Human pathology
- Accession number :
- edsair.doi.dedup.....5b95d30b77eed88371c9b2780d5b9afb