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A TBP–TAF complex required for transcription of human snRNA genes by RNA polymerases II and III
- Source :
- Nature. 374:653-656
- Publication Year :
- 1995
- Publisher :
- Springer Science and Business Media LLC, 1995.
-
Abstract
- The TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs). In general, each of these TBP-TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively. Here we characterize a fourth TBP-TAF complex called SNAPc. Unlike the other TBP-TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein.
- Subjects :
- Transcription, Genetic
Molecular Sequence Data
genetic processes
information science
RNA polymerase II
macromolecular substances
Cross Reactions
RNA Polymerase I
RNA, Small Nuclear
Humans
Amino Acid Sequence
RNA polymerase II holoenzyme
Genetics
Multidisciplinary
Base Sequence
biology
General transcription factor
TATA-Box Binding Protein
DNA
Bacteriophage lambda
TATA Box
Recombinant Proteins
Cell biology
DNA-Binding Proteins
health occupations
biology.protein
Transcription factor II F
RNA Polymerase II
Transcription factor II E
Transcription factor II D
Transcription factor II A
HeLa Cells
Transcription Factors
Subjects
Details
- ISSN :
- 14764687 and 00280836
- Volume :
- 374
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....5b700e80a19c1ce5d6714fce18073ec3