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Rationally designed HIV envelope glycoproteins delivered in a novel adjuvant elicited more broadly reactive antigen-specific antibody responses
- Source :
- Retrovirology, Retrovirology, Vol 9, Iss Suppl 2, p P18 (2012)
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- Methods Here, we designed disulfide-stabilized recombinant HIV1 subtype B (SF162) envelope glycoproteins (Env), gp120 and gp140, by insertion of site-specific cysteine pairs between two layers (layer 1 and 2) in inner domain of gp120. In addition, we identified a novel adjuvant approach using Carbopol 971P, a cross-linked polyanionic carbomer, in combination with the Novartis proprietary oil-in water adjuvant, MF59, to augment humoral immune responses to the Env glycoprotein. We performed thorough in vitro analysis of the disulfide-stabilized Env glycoprotein followed by in vivo evaluations of the adjuvanted-Env glycoprotein boost in rabbits.
- Subjects :
- lcsh:Immunologic diseases. Allergy
viruses
medicine.medical_treatment
MF59
Bioinformatics
law.invention
Immune system
law
In vivo
Virology
medicine
chemistry.chemical_classification
biology
business.industry
virus diseases
Infectious Diseases
chemistry
Poster Presentation
Recombinant DNA
biology.protein
Antibody
lcsh:RC581-607
business
Glycoprotein
Adjuvant
Cysteine
Subjects
Details
- ISSN :
- 17424690
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Retrovirology
- Accession number :
- edsair.doi.dedup.....5b6b170795d4da50d674e634dd34aee9