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Highly selective and sensitive measurement of active forms of FGF21 using novel immunocapture enrichment with LC–MS/MS
- Source :
- Bioanalysis. 10:23-33
- Publication Year :
- 2018
- Publisher :
- Future Science Ltd, 2018.
-
Abstract
- Aim: Recombinant FGF21 analogs are under wide ranging investigations as a potential therapeutic agent for Type 2 diabetes, as well as other metabolic disorders. The endogenous FGF21 is often used as a surrogate pharmacodynamic(PD) biomarker to assess drug efficacy and safety. Results & methodology: Immunocapture was performed using a monoclonal antibody which had been generated to bind to specific domain of native FGF21 as the capture reagent. After immunocapture, enzymatic digestion was performed and a native FGF21-specific tryptic peptide was monitored using LC–MS/MS by selective reaction monitoring. Conclusion: We have successfully developed and validated a bioanalytical assay which provides the specificity to differentiate the endogenous FGF21 from the recombinant therapeutic agent which has nearly identical sequence to the endogenous molecule.
- Subjects :
- 0301 basic medicine
Bioanalysis
medicine.drug_class
Clinical Biochemistry
Endogeny
Monoclonal antibody
Tandem mass spectrometry
Analytical Chemistry
law.invention
03 medical and health sciences
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
law
Lc ms ms
medicine
Humans
General Pharmacology, Toxicology and Pharmaceutics
Chemistry
General Medicine
Biomarker (cell)
Fibroblast Growth Factors
Medical Laboratory Technology
030104 developmental biology
Biochemistry
Recombinant DNA
Chromatography, Liquid
Subjects
Details
- ISSN :
- 17576199 and 17576180
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Bioanalysis
- Accession number :
- edsair.doi.dedup.....5b6aadf5fe4b70b6240fdcf581370f99