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Host macrophage response to injectable hydrogels derived from ECM and α-helical peptides
- Source :
- Mehrban, N, Molina, C P, Quijano, L M, Bowen, J, Johnson, S A, Bartolacci, J, Chang, J, Scott, D J, Woolfson, D N, Birchall, M & Badvlak, S 2020, ' Host Macrophage Response to Injectable Hydrogels Derived From ECM and α-Helical Peptides ', Acta Biomaterialia, vol. 111, pp. 12 . https://doi.org/10.1016/j.actbio.2020.05.022
- Publication Year :
- 2020
-
Abstract
- Tissue engineering materials play a key role in how closely the complex architectural and functional characteristics of native healthy tissue can be replicated. Traditional natural and synthetic materials are superseded by bespoke materials that cross the boundary between these two categories. Here we present hydrogels that are derived from decellularised extracellular matrix and those that are synthesised from de novo α-helical peptides. We assess in vitro activation of murine macrophages to our hydrogels and whether these gels induce an M1-like or M2-like phenotype. This was followed by the in vivo immune macrophage response to hydrogels injected into rat partial-thickness abdominal wall defects. Over 28 days we observe an increase in mononuclear cell infiltration at the hydrogel-tissue interface without promoting a foreign body reaction and see no evidence of hydrogel encapsulation or formation of multinucleate giant cells. We also note an upregulation of myogenic differentiation markers and the expression of anti-inflammatory markers Arginase1, IL-10, and CD206, indicating pro-remodelling for all injected hydrogels. Furthermore, all hydrogels promote an anti-inflammatory environment after an initial spike in the pro-inflammatory phenotype. No difference between the injected site and the healthy tissue is observed after 28 days, indicating full integration. These materials offer great potential for future applications in regenerative medicine and towards unmet clinical needs. STATEMENT OF SIGNIFICANCE: Materials play a key role in how closely the complex architectural and functional characteristics of native healthy tissue can be replicated in tissue engineering. Here we present injectable hydrogels derived from decellularised extracellular matrix and de novo designed α-helical peptides. Over 28 days in the rat abdominal wall we observe an increase in mononuclear cell infiltration at the hydrogel-tissue interface with no foreign body reaction, no evidence of hydrogel encapsulation and no multinucleate giant cells. Our data indicate pro-remodelling and the promotion of an anti-inflammatory environment for all injected hydrogels with evidence of full integration with healthy tissue after 28 days. These unique materials offer great potential for future applications in regenerative medicine and towards designing materials for unmet clinical needs.
- Subjects :
- Macrophage
0206 medical engineering
Biomedical Engineering
BrisSynBio
02 engineering and technology
Biochemistry
Regenerative medicine
complex mixtures
Biomaterials
Extracellular matrix
Mice
Multinucleate
Tissue engineering
In vivo
Animals
Molecular Biology
ECM
Tissue Engineering
Chemistry
Foreign-Body Reaction
Macrophages
Bristol BioDesign Institute
Hydrogels
General Medicine
021001 nanoscience & nanotechnology
020601 biomedical engineering
Cell biology
Extracellular Matrix
Rats
Mononuclear cell infiltration
Giant cell
Peptide
Self-healing hydrogels
synthetic biology
0210 nano-technology
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 17427061
- Database :
- OpenAIRE
- Journal :
- Mehrban, N, Molina, C P, Quijano, L M, Bowen, J, Johnson, S A, Bartolacci, J, Chang, J, Scott, D J, Woolfson, D N, Birchall, M & Badvlak, S 2020, ' Host Macrophage Response to Injectable Hydrogels Derived From ECM and α-Helical Peptides ', Acta Biomaterialia, vol. 111, pp. 12 . https://doi.org/10.1016/j.actbio.2020.05.022
- Accession number :
- edsair.doi.dedup.....5b03c42c4dee844a179d6319e38f5042