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<scp>LncRNA GAS5</scp> inhibits the proliferation and invasion of ovarian clear cell carcinoma via the <scp>miR</scp> ‐31‐5p/ <scp>ARID1A</scp> axis

Authors :
Nian Yang
Yu Huang
Zhongmei Yang
Kana Wang
Yao Xie
Jian Zhang
Source :
Kaohsiung Journal of Medical Sciences, Vol 37, Iss 11, Pp 940-950 (2021)
Publication Year :
2021
Publisher :
Wiley, 2021.

Abstract

To investigate the role of the lncRNA growth arrest special 5 (GAS5) in ovarian clear cell carcinoma (OCCC), we measured the expression of GAS5 and miR‐31‐5p in OCCC tissue samples and OCCC cell lines using RT‐qPCR. MTT and colony formation assays were used to measure cell viability and colony formation ability. Cell invasion was determined by Transwell assays. The binding between GAS5 and miR‐31‐5p as well as miR‐31‐5p and ARID1A was determined by dual‐luciferase reporter assays. The ARID1A protein levels were detected using western blotting. Kaplan–Meier curves were used for the analysis of the 5‐year survival rate of patients with OCCC. GAS5 and ARID1A levels were significantly decreased, while miR‐31‐5p levels were strongly elevated in the OCCC tissues and cell lines. Patients with lower GAS5/ARID1A levels had shorter overall survival times. Overexpression of GAS5 or inhibition of miR‐31‐5p suppressed cell viability and invasion of OCCC cells and upregulated the protein levels of ARID1A. Moreover, overexpression of miR‐31‐5p reversed the effects of overexpression of GAS5. Cotransfection with pcDNA3.1‐GAS5 and miR‐31‐5p inhibitor led to the lowest cell viability and cell invasion rates. A dual‐luciferase reporter assay was performed to confirm the target relationship between GAS5 and miR‐31‐5p, as well as between miR‐31‐5p and ARID1A. LncRNA GAS5 inhibited cell viability and invasion of OCCC through activation of ARID1A by sponging miR‐31‐5p.

Details

ISSN :
24108650 and 1607551X
Volume :
37
Database :
OpenAIRE
Journal :
The Kaohsiung Journal of Medical Sciences
Accession number :
edsair.doi.dedup.....5b00417947126e5e9d38c47736c9e2e0