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Presence of adrenocorticotropin-potentiating factors in porcine thyroid glands
- Source :
- Endocrinologia Japonica. 31:443-449
- Publication Year :
- 1984
- Publisher :
- Japan Endocrine Society, 1984.
-
Abstract
- An extract of porcine thyroid gland in 0.1 N acetic acid exerted dose-dependent potentiation of ACTH-induced corticosterone production in isolated rat adrenal cells. The extract by itself manifested no steroidogenic activity. Upon gel-filtration of the extract, potentiating activities were demonstrated in three main peaks with molecular weights of about 10,000, 5,000 and 2,000. These findings indicate the presence of heterogeneous forms of ACTH-potentiating factors in the thyroid. Significant enhancement of ACTH-induced steroidogenesis was readily apparent with three gel-filtration fractions at a lower concentration of ACTH (4.75 pM). At this concentration, dose-dependent potentiation was observed with these three fractions. Enhanced corticosterone production responses by cells preincubated with the thyroid extract were observed and the results indicated the existence of potentiating mechanisms other than inhibition of ACTH proteolysis. The lack of T4, T3 and thyroglobulin in this activity suggests that the activity resides in other constituents of the thyroid.
- Subjects :
- Thyroid Hormones
endocrine system
medicine.medical_specialty
Swine
medicine.medical_treatment
Thyroid Gland
chemistry.chemical_compound
Acetic acid
Endocrinology
Corticosterone
Internal medicine
Adrenal Glands
medicine
Animals
Dose-Response Relationship, Drug
Molecular mass
Tissue Extracts
Thyroid
General Engineering
Long-term potentiation
Molecular Weight
medicine.drug_formulation_ingredient
medicine.anatomical_structure
chemistry
Chromatography, Gel
Cosyntropin
Thyroglobulin
Thyroid extract
Subjects
Details
- ISSN :
- 21856370 and 00137219
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Endocrinologia Japonica
- Accession number :
- edsair.doi.dedup.....5ad78a81c42af8357f460ba9546a267b