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Sanggenon C Stimulates Osteoblastic Proliferation and Differentiation, Inhibits Osteoclastic Resorption, and Ameliorates Prednisone-Induced Osteoporosis in Zebrafish Model
- Source :
- Molecules, Volume 23, Issue 9, Molecules, Vol 23, Iss 9, p 2343 (2018), Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Sanggenon C (SC), which is a natural flavonoid found in the stem bark of Cortex Mori, has been discovered to have the antioxidant, anti-inflammatory, and antitumor properties. However, its effect in osteoporosis has not yet been reported. In this research, the effect of SC on the proliferation of MC3T3-E1 cells was evaluated by using the MTT assay. Alkaline phosphatase (ALP) activity and the mRNA expression of Runx2, Collagen I, OPG, and RANKL were examined. TRAP-positive cell counting and bone resorption pits were adopted to observe the effect of SC on the formation and function of osteoclasts. Next, the mRNA level of TRAP, CTSK, NFATc1, and TRAF6 of osteoclasts were measured by real-time qPCR. In addition, the anti-osteoporosis activity of SC in vivo was evaluated in the zebrafish model. Our study indicated that SC exhibited a significant stimulatory effect on MC3T3-E1 cell proliferation at 1 to 10 &mu<br />M and caused an increase in ALP activity at 0.3 to 10 &mu<br />M. It could upregulate the expression of Runx2, Collagen I, and increases the OPG/RANKL ratio. Furthermore, SC was found to inhibit the formation and function of osteoclasts, which is demonstrated by a lower number of TRAP-positive multinuclear cells and a fewer area of bone resorption pits compared to the control group. TRAP, CTSK, and NFATc1 were downregulated in 0.3 to 10 &mu<br />M SC treated groups. In addition, 3 to 10 &mu<br />M SC also inhibited the expression of TRAF6 mRNA. When prednisone-induced zebrafish was treated with 0.3, 1, 3, and 10 &mu<br />M SC, higher mineralization of vertebrate column was discovered in a dose-dependent pattern, which suggests that SC could reverse the bone loss of zebrafish caused by prednisone. In summary, these findings indicated that SC has the potential to prevent or treat osteoporosis.
- Subjects :
- 0301 basic medicine
Osteoclasts
Pharmaceutical Science
Sanggenon C
Analytical Chemistry
Mice
0302 clinical medicine
Drug Discovery
biology
Chemistry
Cell Differentiation
Osteoblast
medicine.anatomical_structure
Chemistry (miscellaneous)
RANKL
030220 oncology & carcinogenesis
osteoclast
osteoblast
Molecular Medicine
Alkaline phosphatase
Collagen
musculoskeletal diseases
Article
Bone resorption
Cell Line
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Osteoclast
In vivo
medicine
Animals
MTT assay
Physical and Theoretical Chemistry
Benzofurans
Cell Proliferation
Osteoblasts
Cell growth
RANK Ligand
Organic Chemistry
Osteoprotegerin
Core Binding Factor alpha Subunits
Alkaline Phosphatase
zebrafish
Molecular biology
osteoporosis
Disease Models, Animal
030104 developmental biology
Gene Expression Regulation
Chromones
biology.protein
Prednisone
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....5acc2b2852440b4715e7519a31eee375
- Full Text :
- https://doi.org/10.3390/molecules23092343