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Analysis of Alpha-Synuclein in Malignant Melanoma - Development of a SRM Quantification Assay
- Source :
- PLoS ONE; 9(10), no e110804 (2014), PLoS ONE, PLoS ONE, Vol 9, Iss 10, p e110804 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Globally, malignant melanoma shows a steady increase in the incidence among cancer diseases. Malignant melanoma represents a cancer type where currently no biomarker or diagnostics is available to identify disease stage, progression of disease or personalized medicine treatment. The aim of this study was to assess the tissue expression of alpha-synuclein, a protein implicated in several disease processes, in metastatic tissues from malignant melanoma patients. A targeted Selected Reaction Monitoring (SRM) assay was developed and utilized together with stable isotope labeling for the relative quantification of two target peptides of alpha-synuclein. Analysis of alpha-synuclein protein was then performed in ten metastatic tissue samples from the Lund Melanoma Biobank. The calibration curve using peak area ratio (heavy/light) versus concentration ratios showed linear regression over three orders of magnitude, for both of the selected target peptide sequences. In support of the measurements of specific protein expression levels, we also observed significant correlation between the protein and mRNA levels of alpha-synuclein in these tissues. Investigating levels of tissue alpha-synuclein may add novel aspect to biomarker development in melanoma, help to understand disease mechanisms and ultimately contribute to discriminate melanoma patients with different prognosis.
- Subjects :
- Proteomics
Melanomas
Male
Pathology
lcsh:Medicine
Biochemistry
Mass Spectrometry
Metastasis
Analytical Chemistry
Basic Cancer Research
Medicine and Health Sciences
Neoplasm Metastasis
lcsh:Science
Melanoma
Aged, 80 and over
Multidisciplinary
Middle Aged
Prognosis
Orders of magnitude (mass)
Gene Expression Regulation, Neoplastic
Chemistry
Oncology
Liquid Chromatography-Tandem Mass Spectrometry
Organ Specificity
Physical Sciences
alpha-Synuclein
Biomarker (medicine)
Female
Anatomy
Research Article
Adult
medicine.medical_specialty
Lymphoid Tissue
Biology
Lymphatic System
Malignant Tumors
Biomarkers, Tumor
medicine
Humans
Synthetic Peptides
RNA, Messenger
Aged
business.industry
lcsh:R
Biology and Life Sciences
Cancers and Neoplasms
Cancer
medicine.disease
Cancer and Oncology
Cutaneous melanoma
Cancer research
lcsh:Q
Personalized medicine
Peptides
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE; 9(10), no e110804 (2014), PLoS ONE, PLoS ONE, Vol 9, Iss 10, p e110804 (2014)
- Accession number :
- edsair.doi.dedup.....5abbea0d366d5322466d77cef8a8497a