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Improved lyophilization conditions for long-term storage of bacteriophages
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group UK, 2019.
-
Abstract
- Phage therapy is one of the promising alternatives to combat the increasing problem of antibiotic resistance. Lyophilization is used for the preparation of pharmaceutical products to improve their stability in long-term storage. The aim of this study was to improve the stability of lyophilized bacteriophages using different excipients. Three lytic bacteriophages Escherichia phage ECP311, Klebsiella phage KPP235 and Enterobacter phage ELP140 were subjected to lyophilization using six different excipients: glucose, sucrose, gelatin, mannitol, polyethylene glycol and sorbitol. The lyophilized phages were stored at 4 °C and 37 °C and rehydrated using biological saline to test their viability at 5 months interval up to 20 months. The results showed that the use of sucrose, gelatin and their combination was beneficial in maintaining the viability of phages post-lyophilization. When lyophilized phages were stored at 4 °C, their viability was maintained up to 20 months, but at 37 °C there was a reduction in activity after 10 months. This is one of the few studies to report the lyophilization of phage cocktails to have viability for up to 10 months. Our study identified promising lyophilization excipients to effectively lyophilize bacteriophages for pharmaceutical applications and long-term storage.
- Subjects :
- 0301 basic medicine
Sucrose
food.ingredient
Phage therapy
medicine.medical_treatment
030106 microbiology
Enterobacter
lcsh:Medicine
Phage biology
Polyethylene glycol
Gelatin
Coliphages
Article
Excipients
03 medical and health sciences
chemistry.chemical_compound
food
Klebsiella
medicine
Humans
Bacteriophages
Food science
Phage Therapy
lcsh:Science
Multidisciplinary
biology
lcsh:R
Temperature
biology.organism_classification
030104 developmental biology
Freeze Drying
chemistry
Lytic cycle
lcsh:Q
Sorbitol
Mannitol
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....5a8284c21f4cf1001f21b462de4db093