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Modeling the fate of antibiotic resistance genes and class 1 integrons during thermophilic anaerobic digestion of municipal wastewater solids
- Source :
- Applied Microbiology and Biotechnology. 100:1437-1444
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- This study investigated the use of thermophilic anaerobic digestion for removing antibiotic resistance genes (ARGs) from residual municipal wastewater solids. Four laboratory-scale anaerobic digesters were operated in 8-day batch cycles at temperatures of 40, 56, 60, and 63 °C. Two tetracycline resistance genes (tet(W) and tet(X)), a fluoroquinolone resistance gene (qnrA), the integrase gene of class 1 integrons (intI1), 16S rRNA genes of all Bacteria, and 16S rRNA genes of methanogens were quantified using real-time quantitative PCR. ARG and intI1 quantities decreased at all temperatures and were described well by a modified form of the Collins-Selleck disinfection kinetic model. The magnitudes of Collins-Selleck kinetic parameters were significantly greater at thermophilic temperatures compared to 40 °C, but few statistically significant differences were observed among these parameters for the thermophilic anaerobic digesters. This model allows for the direct comparison of different operating conditions (e.g., temperature) on anaerobic digestion performance in mitigating the quantity of ARGs in wastewater solids and could be used to design full-scale anaerobic digesters to specifically treat for ARGs as a "pollutant" of concern.
- Subjects :
- 0301 basic medicine
Pollutant
biology
Tetracycline
Thermophile
030106 microbiology
General Medicine
010501 environmental sciences
biology.organism_classification
16S ribosomal RNA
01 natural sciences
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Anaerobic digestion
Wastewater
medicine
Food science
Anaerobic exercise
Bacteria
0105 earth and related environmental sciences
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....5a6a8305c64719077c31a6bda631066c