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Influence of different uropathogenic microorganisms on human sperm motility parameters in an in vitro experiment
- Source :
- Andrologia. 30
- Publication Year :
- 1998
-
Abstract
- Summary. The influence of different uropathogenic microorganisms (E. coli, enterococcus, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Candida albicans) on human sperm motility was studied in vitro with a computer-assisted sperm analyser (CASA). Native ejaculates were prepared with the swim-up technique and adjusted to 22 times 106 spermatozoa ml−1. The sperm suspension was artificially infected with microorganisms in concentrations varying from 2 times 103 to 2 times 107. Sperm motility was examined directly after incubation, 2, 4 and 6 h later using the Mika motion analysis®, a computer-based, automatic motility analysis. Former results with E. coli (serotype 06) could be confirmed that a significant inhibitory effect on sperm motility was associated with bacterial growth. Experiments with the enterococcus strain and Staphylococcus saprophyticus indicated no significant influence on sperm motility parameters. Tests with Pseudomonas aeruginosa showed a decrease of progressive motility according to time, but not to different bacterial concentrations. A significant inhibitory effect of Candida albicans was only detected in the samples with the initial bacterial concentration of 2 times 107 microorganisms ml−1.
- Subjects :
- Male
Urology
Staphylococcus
Motility
Bacterial growth
In Vitro Techniques
medicine.disease_cause
Microbiology
Endocrinology
Male Urogenital Diseases
Candida albicans
medicine
Escherichia coli
Humans
Sperm motility
Infertility, Male
Staphylococcus saprophyticus
biology
Bacteria
Pseudomonas aeruginosa
Candidiasis
General Medicine
Bacterial Infections
biology.organism_classification
Sperm
Sperm Motility
Enterococcus
Subjects
Details
- ISSN :
- 03034569
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Andrologia
- Accession number :
- edsair.doi.dedup.....5a679292e66b8dbca111213ea2a545a8