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Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species
- Source :
- Genetic resources and crop evolution, 57 (2010): 533–542. doi:10.1007/s10722-009-9491-6, info:cnr-pdr/source/autori:Lioi L., Galasso I., Daminati M.G., Piergiovanni A.R./titolo:Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species/doi:10.1007%2Fs10722-009-9491-6/rivista:Genetic resources and crop evolution (Print)/anno:2010/pagina_da:533/pagina_a:542/intervallo_pagine:533–542/volume:57
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- The primary structure of the double-headed Bowman-Birk inhibitor (BBI) family and the antitryptic activity were investigated in cultivated and wild Phaseolus species. Two BBI types were identified; the first one inhibits trypsin and chymotrypsin (tc-BBI), the second one elastase and trypsin (et-BBI). Only tc-BBI was found in P. lunatus and P. parvulus, while none of BBI types, identified in this study, was found in P. leptostachyus. The deduced amino acid sequences revealed some polymorphisms within both tc-BBI and et-BBI binding loops that could affect the inhibitory activity. The trypsin inhibitor content showed a high variation with the lowest value recorded in P. lepthostachyus and the highest one observed in P. oligospermus. Southern blot analysis confirmed the absence of both BBI types in P. leptostachyus and suggests that in P. coccineus, P. dumosus and P. costaricensis, the two genes were clustered in a narrow genomic region of 1.3kbp.
- Subjects :
- Trypsin inhibitor
Plant Science
Gene organization
Genetics
medicine
Inhibitor domain polymorphism
Ecology, Evolution, Behavior and Systematics
Southern blot
Phaseolus
chemistry.chemical_classification
Chymotrypsin
Kunitz STI protease inhibitor
biology
Protein primary structure
biology.organism_classification
Trypsin
Molecular biology
Amino acid
Antitryptic activity
chemistry
Biochemistry
biology.protein
Wild bean
Agronomy and Crop Science
medicine.drug
Subjects
Details
- ISSN :
- 15735109 and 09259864
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Genetic Resources and Crop Evolution
- Accession number :
- edsair.doi.dedup.....5a5f35904ba6c82f507f1d5cb1472031