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Differential role of TNF-alpha and IFN-gamma in the brain of rats with chronic relapsing autoimmune encephalomyelitis
- Source :
- Journal of neuroimmunology. 96(1)
- Publication Year :
- 1999
-
Abstract
- To elucidate the mechanisms of relapses of the clinical signs in experimental autoimmune encephalomyelitis (EAE), the cytokine profile of chronic relapsing EAE (CR-EAE) in rats was determined by competitive polymerase chain reaction (PCR). By immunization with guinea pig spinal cord homogenate and treatment with low-dose cyclosporin A (CsA), rats developed two attacks of EAE with remission in between. Cytokine analysis revealed that the level of TNF-alpha mRNA increased at the first and second attacks with transient disappearance at the remission phase. In contrast, the level of IFN-gamma mRNA was suppressed at the first attack by CsA and peaked at the second attack. Intraventricular administration of IFN-gamma prior to onset of disease signs induced more relapses, or a severe lethal form. In addition, the intraventricular injection of TNF-alpha caused the persistence of the clinical signs. These findings suggest that TNF-alpha contributes to the first and second attacks of CR-EAE, while IFN-gamma is not required for the first attack but is closely related to the relapse of the disease. With regard to anti-inflammatory cytokines, the levels of both TGF-beta1 and IL-10 mRNA at the second attack were higher than those at the first attack. Taken together, differential involvement of TNF-alpha and IFN-gamma is closely associated with the clinical features of CR-EAE.
- Subjects :
- Male
Encephalomyelitis, Autoimmune, Experimental
medicine.medical_treatment
Immunology
Guinea Pigs
Gene Expression
Disease
Polymerase Chain Reaction
Guinea pig
Interferon-gamma
Recurrence
Transforming Growth Factor beta
Cyclosporin a
medicine
Immunology and Allergy
Animals
RNA, Messenger
Injections, Intraventricular
Messenger RNA
business.industry
Tumor Necrosis Factor-alpha
Experimental autoimmune encephalomyelitis
Brain
medicine.disease
Spinal cord
Interleukin-10
Rats
medicine.anatomical_structure
Cytokine
Neurology
Immunization
Spinal Cord
Rats, Inbred Lew
Chronic Disease
Cyclosporine
Female
Neurology (clinical)
business
Immunosuppressive Agents
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 96
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of neuroimmunology
- Accession number :
- edsair.doi.dedup.....5a5e72d8802d666c46a826c7fdeb5d60