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Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemia cells
- Source :
- Blood. 78:2962-2968
- Publication Year :
- 1991
- Publisher :
- American Society of Hematology, 1991.
-
Abstract
- The c-kit proto-oncogene encodes a receptor tyrosine kinase that is thought to play an important role in hematopoiesis. In a series of human acute myeloblastic leukemia (AML), the expression of the c-kit proto-oncogene and its product was studied by means of Northern blot and immunoblot analyses. The c-kit mRNA was expressed in 20 of 25 cases of AML, and in those cases the product of the c-kit proto-oncogene was detected by immunoblotting with anti-c-kit antibody. The expression of c-kit transcripts and protein was barely detectable in normal bone marrow cells as a control. The expression of c-kit transcript did not correlate with the French-American-British classification nor clinical manifestations. In 6 of 11 cases that expressed c-kit product, AML cells were found to proliferate in response to recombinant human stem cell factor (rhSCF), the ligand for c-kit, and the synergistic stimulation of AML cells was observed by rhSCF and granulocyte- macrophage colony-stimulating factor. Immunoblotting with anti- phosphotyrosine antibody showed that the c-kit receptor protein was detectably phosphorylated in 7 of 12 cases tested before the stimulation with rhSCF, while the rhSCF treatment resulted in an increased tyrosine phosphorylation of c-kit in AML cells. These results indicate that c-kit proto-oncogene is expressed in most cases of AML and is functional in terms of supporting proliferation.
- Subjects :
- Acute myeloblastic leukemia
Blotting, Western
Immunology
Gene Expression
In Vitro Techniques
Hematopoietic Cell Growth Factors
Proto-Oncogene Mas
Biochemistry
Recombinant Human Stem Cell Factor
Receptor tyrosine kinase
Proto-Oncogene Proteins
Proto-Oncogenes
Tumor Cells, Cultured
medicine
Humans
RNA, Messenger
RNA, Neoplasm
Northern blot
Phosphorylation
Phosphotyrosine
Receptor
Stem Cell Factor
biology
Cell Biology
Hematology
Blotting, Northern
medicine.disease
Molecular biology
Recombinant Proteins
Leukemia, Myeloid, Acute
Proto-Oncogene Proteins c-kit
Haematopoiesis
biology.protein
Tyrosine
Antibody
Cell Division
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....5a5714beb471ff07e71ce1da39cf3088