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A Microfluidic Device for Rapid Screening ofE. coliO157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis
- Source :
- Chemistry - A European Journal. 23:12754-12757
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by sur face tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre-concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point-of-need microbiological water quality monitoring.
- Subjects :
- Light
02 engineering and technology
Firefly Luciferin
Wastewater
Escherichia coli O157
Immunomagnetic separation
medicine.disease_cause
01 natural sciences
Catalysis
law.invention
Adenosine Triphosphate
law
Lab-On-A-Chip Devices
medicine
Surface Tension
Bioluminescence
Luciferase
Luciferases
Effluent
Escherichia coli
Filtration
Colony-forming unit
Chromatography
Chemistry
010401 analytical chemistry
Organic Chemistry
General Chemistry
021001 nanoscience & nanotechnology
Luciferin
Molecular biology
0104 chemical sciences
Luminescent Measurements
0210 nano-technology
Subjects
Details
- ISSN :
- 09476539
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Chemistry - A European Journal
- Accession number :
- edsair.doi.dedup.....5a56c569e52ffb9f762c077a7b1634a4