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p300-Mediated Histone Acetylation is Essential for the Regulation of GATA4 and MEF2C by BMP2 in H9c2 Cells
- Source :
- Cardiovascular Toxicology. 13:316-322
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Histone acetylase (HAT) p300 plays an important role in the regulation of cardiac gene expression. During cardiac development, bone morphogenetic protein (BMP)-2 induces the expression of cardiac transcription factors. However, the underlying molecular mechanism(s) is not clear. In the present study, we tested the hypothesis that p300-mediated histone acetylation was essential for the regulation of cardiac transcription factors by BMP2. Cultured rat H9c2 embryonic cardiac myocytes (H9c2 cells) were transfected with recombinant adenoviruses expressing human BMP2 (AdBMP2) with or without curcumin, a specific p300-HAT inhibitor. Quantitative real-time RT-PCR analysis showed that curcumin substantially inhibited both AdBMP2-induced and basal expression levels of cardiac transcription factors GATA4 and MEF2C, but not Tbx5. Similarly, chromatin immunoprecipitation (ChIP) analysis showed that curcumin inhibited both AdBMP2-induced and basal histone H3 acetylation levels in the promoter regions of GATA4 and MEF2C, but not of Tbx5. In addition, curcumin selectively suppressed AdBMP2-induced expression of HAT p300, but not HAT GCN5 in H9c2 cells. The data indicated that inhibition of histone H3 acetylation with curcumin diminished BMP2-induced expression of GATA4 and MEF2C, suggesting that p300-mediated histone acetylation was essential for the regulation of GATA4 and MEF2C by BMP2 in H9c2 cells.
- Subjects :
- Curcumin
Bone Morphogenetic Protein 2
Biology
SAP30
Transfection
Toxicology
Histones
Histone H2A
Animals
Humans
Histone H3 acetylation
Molecular Biology
Cells, Cultured
MEF2 Transcription Factors
Acetylation
HDAC4
Molecular biology
GATA4 Transcription Factor
Rats
Histone
Histone methyltransferase
embryonic structures
cardiovascular system
biology.protein
Cardiology and Cardiovascular Medicine
E1A-Associated p300 Protein
Chromatin immunoprecipitation
Subjects
Details
- ISSN :
- 15590259 and 15307905
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Cardiovascular Toxicology
- Accession number :
- edsair.doi.dedup.....5a53029ccb4e4c2385778f4ce5a0b767